The kinase activity of pseudorabies virus US3 is required for modulation of the actin cytoskeleton

Céline Van den Broeke; Matthias Deruelle; Hans J. Nauwynck; Kelly E. Coller; Gregory A. Smith; Jan Van Doorsselaere; Herman W. Favoreel

doi:10.1016/j.virol.2008.11.050

The kinase activity of pseudorabies virus US3 is required for modulation of the actin cytoskeleton

Céline Van den Broeke, Matthias Deruelle, Hans J. Nauwynck, Kelly E. Coller, Gregory A. Smith, Jan Van Doorsselaere, Herman W. Favoreel^*

^*Corresponding author for this work

Microbiology-Immunology

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Different viruses exploit the host cytoskeleton to facilitate replication and spread. The conserved US3 protein of the alphaherpesvirus pseudorabies virus induces actin stress fiber disassembly and formation of actin-containing cell projections, which are associated with enhanced intercellular virus spread. Proteins of members of other virus families, notably vaccinia virus F11L protein and human immunodeficiency virus Nef protein, induce actin rearrangements that are very similar to those induced by US3. Interestingly, unlike F11L and Nef, the US3 protein displays serine/threonine kinase activity. Here, we report that the kinase activity of pseudorabies virus US3 is absolutely required for its actin modulating activity. These data show that different viruses have developed independent mechanisms to induce very similar actin rearrangements.

Original language	English (US)
Pages (from-to)	155-160
Number of pages	6
Journal	Virology
Volume	385
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2008.11.050
State	Published - Mar 1 2009

Funding

This research was supported by a research grant of the Research Foundation - Flanders (FWO-Vlaanderen, grant nr. G.0196.06), a Concerted Research Action of Ghent University and National Institutes of Health grant 1RO1AI056346 (to G.A.S.). We would like to thank Carine Boonen, Natasha De Corte, and Lieve Sys for excellent technical assistance, Jenifer Klabis for performing the RFLP assays and sequencing PCRs, and L.A. Olsen and L. Enquist for US3-specific antibodies.

Keywords

Actin
Cell projections
Herpesvirus
Kinase activity
Pseudorabies virus
Stress fibers
US3

ASJC Scopus subject areas

Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virol.2008.11.050

Cite this

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title = "The kinase activity of pseudorabies virus US3 is required for modulation of the actin cytoskeleton",

abstract = "Different viruses exploit the host cytoskeleton to facilitate replication and spread. The conserved US3 protein of the alphaherpesvirus pseudorabies virus induces actin stress fiber disassembly and formation of actin-containing cell projections, which are associated with enhanced intercellular virus spread. Proteins of members of other virus families, notably vaccinia virus F11L protein and human immunodeficiency virus Nef protein, induce actin rearrangements that are very similar to those induced by US3. Interestingly, unlike F11L and Nef, the US3 protein displays serine/threonine kinase activity. Here, we report that the kinase activity of pseudorabies virus US3 is absolutely required for its actin modulating activity. These data show that different viruses have developed independent mechanisms to induce very similar actin rearrangements.",

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note = "Funding Information: This research was supported by a research grant of the Research Foundation - Flanders (FWO-Vlaanderen, grant nr. G.0196.06), a Concerted Research Action of Ghent University and National Institutes of Health grant 1RO1AI056346 (to G.A.S.). We would like to thank Carine Boonen, Natasha De Corte, and Lieve Sys for excellent technical assistance, Jenifer Klabis for performing the RFLP assays and sequencing PCRs, and L.A. Olsen and L. Enquist for US3-specific antibodies.",

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AU - Smith, Gregory A.

AU - Van Doorsselaere, Jan

AU - Favoreel, Herman W.

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PY - 2009/3/1

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N2 - Different viruses exploit the host cytoskeleton to facilitate replication and spread. The conserved US3 protein of the alphaherpesvirus pseudorabies virus induces actin stress fiber disassembly and formation of actin-containing cell projections, which are associated with enhanced intercellular virus spread. Proteins of members of other virus families, notably vaccinia virus F11L protein and human immunodeficiency virus Nef protein, induce actin rearrangements that are very similar to those induced by US3. Interestingly, unlike F11L and Nef, the US3 protein displays serine/threonine kinase activity. Here, we report that the kinase activity of pseudorabies virus US3 is absolutely required for its actin modulating activity. These data show that different viruses have developed independent mechanisms to induce very similar actin rearrangements.

AB - Different viruses exploit the host cytoskeleton to facilitate replication and spread. The conserved US3 protein of the alphaherpesvirus pseudorabies virus induces actin stress fiber disassembly and formation of actin-containing cell projections, which are associated with enhanced intercellular virus spread. Proteins of members of other virus families, notably vaccinia virus F11L protein and human immunodeficiency virus Nef protein, induce actin rearrangements that are very similar to those induced by US3. Interestingly, unlike F11L and Nef, the US3 protein displays serine/threonine kinase activity. Here, we report that the kinase activity of pseudorabies virus US3 is absolutely required for its actin modulating activity. These data show that different viruses have developed independent mechanisms to induce very similar actin rearrangements.

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The kinase activity of pseudorabies virus US3 is required for modulation of the actin cytoskeleton

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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