Idiotype analyses of plaque‐forming cells using anti‐idiotype antibody against TEPC‐ 15 myeloma protein (anti‐T 15 id) indicated that the proportion of plaque‐forming cells producing anti‐phosphorylcholine (PC) antibodies with idiotypic determinants of T15id varied depending on the strain of mice (15–97%). However, treatment of neonates of those mouse strains with the anti‐T 15 id antibody rendered them unresponsive to subsequent stimulation with PC‐containing antigen (<7% of control response). The treatment of spleen cell cultures, derived from adult mice, with anti‐ T15 id antibody resulted in complete suppression of T15 id, although the suppression of the total anti‐PC response was much less pronounced as compared to that induced in vivo. These results suggest that anti‐T 15 id antibodies injected in the neonatal period may chronically inhibit the differentiation of B lymphocytes specific for PC. The lack of compensatory increases in the production of anti‐PC antibodies bearing other idiotypes in T15 id‐suppressed mice does not appear to be related to the clonal proportion of cells producing anti‐PC with non‐T 15 id. This tolerance induction by anti‐idiotype antibody appears to be unique to the nature of clonal differentiation of anti‐PC‐producing lymphocytes.
ASJC Scopus subject areas
- Immunology and Allergy