The long non-coding RNA BBOX1 antisense RNA 1 is upregulated in polycystic ovary syndrome (PCOS) and suppresses the role of microRNA-19b in the proliferation of ovarian granulose cells: Short title: BBOX1 antisense RNA 1 in cell proliferation

Zhi Zhou; Yong Zhang; Can Tan; Juan Zhang; Guohui Yi; Bangbei Wang; Yejuan Li; Hui Lu; Weiying Lu; Xiaopo Zhang

doi:10.1186/s12905-023-02632-5

The long non-coding RNA BBOX1 antisense RNA 1 is upregulated in polycystic ovary syndrome (PCOS) and suppresses the role of microRNA-19b in the proliferation of ovarian granulose cells: Short title: BBOX1 antisense RNA 1 in cell proliferation

Zhi Zhou, Yong Zhang, Can Tan, Juan Zhang, Guohui Yi, Bangbei Wang, Yejuan Li, Hui Lu, Weiying Lu^*, Xiaopo Zhang^*

^*Corresponding author for this work

Medicine, Cardiology Division

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Background: MicroRNA-19b (miR-19b) has been reported to be downregulated in polycystic ovary syndrome (PCOS), while its upstream regulators are unclear. We speculated that miR-19b could potentially form a binding relationship with BBOX1 antisense RNA 1 (BBOX1-AS1), a long non-coding RNA recognized for its critical role in ovarian cancer. Subsequently, we investigated into their interaction in PCOS. Methods: The expression of miR-19b and BBOX1-AS1 in follicular fluid from both control women (n = 80) and women with PCOS (n = 80) was detected by RT-qPCR. Correlations were analyzed with Pearson’ correlation coefficient. The binding of miR-19b to the wild-type (-wt) ad mutant (-mut) BBOX1-AS1 was determined by RNA-RNA pulldown assay. Their interactions were detected by overexpression assay. Bromodeoxyuridine (BrdU) assay was applied for proliferation analysis. Results: BBOX1-AS1 was highly upregulated, while miR-19b was downregulated in PCOS. There was no close correlation across PCOS and the control samples. Consistently, they did not regulate the expression of each other in granulosa cells. However, BBOX1-AS1-wt, but not BBOX1-AS1-mut, could directly interact with miR-19b. BBOX1-AS1 suppressed the role of miR-19b in inhibiting granulosa cell proliferation. Conclusion: BBOX1-AS1 is highly upregulated in PCOS, and it may serve as an endogenous competing RNA for miR-19b to suppress its role in inhibiting granulosa cell proliferation. Our study suggested the role of BBOX1-AS1 as a potential target to treat PCOS.

Original language	English (US)
Article number	508
Journal	BMC Women's Health
Volume	23
Issue number	1
DOIs	https://doi.org/10.1186/s12905-023-02632-5
State	Published - Dec 2023

Funding

This study was supported by Science and Technology Department of Hainan Province (2019RC389, ZDYF2017086, ZDYF2019157) and Hainan Province Clinical Medical Center (QWYH202175).

Keywords

BBOX1-AS1
Granulosa cell proliferation
Polycystic ovary syndrome
miR-19b

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Zhou, Z., Zhang, Y., Tan, C., Zhang, J., Yi, G., Wang, B., Li, Y., Lu, H., Lu, W., & Zhang, X. (2023). The long non-coding RNA BBOX1 antisense RNA 1 is upregulated in polycystic ovary syndrome (PCOS) and suppresses the role of microRNA-19b in the proliferation of ovarian granulose cells: Short title: BBOX1 antisense RNA 1 in cell proliferation. BMC Women's Health, 23(1), Article 508. https://doi.org/10.1186/s12905-023-02632-5

@article{a4dc21d2e00044ba954befeb9bcaeac3,

title = "The long non-coding RNA BBOX1 antisense RNA 1 is upregulated in polycystic ovary syndrome (PCOS) and suppresses the role of microRNA-19b in the proliferation of ovarian granulose cells: Short title: BBOX1 antisense RNA 1 in cell proliferation",

abstract = "Background: MicroRNA-19b (miR-19b) has been reported to be downregulated in polycystic ovary syndrome (PCOS), while its upstream regulators are unclear. We speculated that miR-19b could potentially form a binding relationship with BBOX1 antisense RNA 1 (BBOX1-AS1), a long non-coding RNA recognized for its critical role in ovarian cancer. Subsequently, we investigated into their interaction in PCOS. Methods: The expression of miR-19b and BBOX1-AS1 in follicular fluid from both control women (n = 80) and women with PCOS (n = 80) was detected by RT-qPCR. Correlations were analyzed with Pearson{\textquoteright} correlation coefficient. The binding of miR-19b to the wild-type (-wt) ad mutant (-mut) BBOX1-AS1 was determined by RNA-RNA pulldown assay. Their interactions were detected by overexpression assay. Bromodeoxyuridine (BrdU) assay was applied for proliferation analysis. Results: BBOX1-AS1 was highly upregulated, while miR-19b was downregulated in PCOS. There was no close correlation across PCOS and the control samples. Consistently, they did not regulate the expression of each other in granulosa cells. However, BBOX1-AS1-wt, but not BBOX1-AS1-mut, could directly interact with miR-19b. BBOX1-AS1 suppressed the role of miR-19b in inhibiting granulosa cell proliferation. Conclusion: BBOX1-AS1 is highly upregulated in PCOS, and it may serve as an endogenous competing RNA for miR-19b to suppress its role in inhibiting granulosa cell proliferation. Our study suggested the role of BBOX1-AS1 as a potential target to treat PCOS.",

keywords = "BBOX1-AS1, Granulosa cell proliferation, Polycystic ovary syndrome, miR-19b",

author = "Zhi Zhou and Yong Zhang and Can Tan and Juan Zhang and Guohui Yi and Bangbei Wang and Yejuan Li and Hui Lu and Weiying Lu and Xiaopo Zhang",

note = "Publisher Copyright: {\textcopyright} 2023, BioMed Central Ltd., part of Springer Nature.",

year = "2023",

month = dec,

doi = "10.1186/s12905-023-02632-5",

language = "English (US)",

volume = "23",

journal = "BMC Women's Health",

issn = "1472-6874",

publisher = "BioMed Central",

number = "1",

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Zhou, Z, Zhang, Y, Tan, C, Zhang, J, Yi, G, Wang, B, Li, Y, Lu, H, Lu, W & Zhang, X 2023, 'The long non-coding RNA BBOX1 antisense RNA 1 is upregulated in polycystic ovary syndrome (PCOS) and suppresses the role of microRNA-19b in the proliferation of ovarian granulose cells: Short title: BBOX1 antisense RNA 1 in cell proliferation', BMC Women's Health, vol. 23, no. 1, 508. https://doi.org/10.1186/s12905-023-02632-5

The long non-coding RNA BBOX1 antisense RNA 1 is upregulated in polycystic ovary syndrome (PCOS) and suppresses the role of microRNA-19b in the proliferation of ovarian granulose cells: Short title: BBOX1 antisense RNA 1 in cell proliferation. / Zhou, Zhi; Zhang, Yong; Tan, Can et al.
In: BMC Women's Health, Vol. 23, No. 1, 508, 12.2023.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - The long non-coding RNA BBOX1 antisense RNA 1 is upregulated in polycystic ovary syndrome (PCOS) and suppresses the role of microRNA-19b in the proliferation of ovarian granulose cells

T2 - Short title: BBOX1 antisense RNA 1 in cell proliferation

AU - Zhou, Zhi

AU - Zhang, Yong

AU - Tan, Can

AU - Zhang, Juan

AU - Yi, Guohui

AU - Wang, Bangbei

AU - Li, Yejuan

AU - Lu, Hui

AU - Lu, Weiying

AU - Zhang, Xiaopo

PY - 2023/12

Y1 - 2023/12

N2 - Background: MicroRNA-19b (miR-19b) has been reported to be downregulated in polycystic ovary syndrome (PCOS), while its upstream regulators are unclear. We speculated that miR-19b could potentially form a binding relationship with BBOX1 antisense RNA 1 (BBOX1-AS1), a long non-coding RNA recognized for its critical role in ovarian cancer. Subsequently, we investigated into their interaction in PCOS. Methods: The expression of miR-19b and BBOX1-AS1 in follicular fluid from both control women (n = 80) and women with PCOS (n = 80) was detected by RT-qPCR. Correlations were analyzed with Pearson’ correlation coefficient. The binding of miR-19b to the wild-type (-wt) ad mutant (-mut) BBOX1-AS1 was determined by RNA-RNA pulldown assay. Their interactions were detected by overexpression assay. Bromodeoxyuridine (BrdU) assay was applied for proliferation analysis. Results: BBOX1-AS1 was highly upregulated, while miR-19b was downregulated in PCOS. There was no close correlation across PCOS and the control samples. Consistently, they did not regulate the expression of each other in granulosa cells. However, BBOX1-AS1-wt, but not BBOX1-AS1-mut, could directly interact with miR-19b. BBOX1-AS1 suppressed the role of miR-19b in inhibiting granulosa cell proliferation. Conclusion: BBOX1-AS1 is highly upregulated in PCOS, and it may serve as an endogenous competing RNA for miR-19b to suppress its role in inhibiting granulosa cell proliferation. Our study suggested the role of BBOX1-AS1 as a potential target to treat PCOS.

AB - Background: MicroRNA-19b (miR-19b) has been reported to be downregulated in polycystic ovary syndrome (PCOS), while its upstream regulators are unclear. We speculated that miR-19b could potentially form a binding relationship with BBOX1 antisense RNA 1 (BBOX1-AS1), a long non-coding RNA recognized for its critical role in ovarian cancer. Subsequently, we investigated into their interaction in PCOS. Methods: The expression of miR-19b and BBOX1-AS1 in follicular fluid from both control women (n = 80) and women with PCOS (n = 80) was detected by RT-qPCR. Correlations were analyzed with Pearson’ correlation coefficient. The binding of miR-19b to the wild-type (-wt) ad mutant (-mut) BBOX1-AS1 was determined by RNA-RNA pulldown assay. Their interactions were detected by overexpression assay. Bromodeoxyuridine (BrdU) assay was applied for proliferation analysis. Results: BBOX1-AS1 was highly upregulated, while miR-19b was downregulated in PCOS. There was no close correlation across PCOS and the control samples. Consistently, they did not regulate the expression of each other in granulosa cells. However, BBOX1-AS1-wt, but not BBOX1-AS1-mut, could directly interact with miR-19b. BBOX1-AS1 suppressed the role of miR-19b in inhibiting granulosa cell proliferation. Conclusion: BBOX1-AS1 is highly upregulated in PCOS, and it may serve as an endogenous competing RNA for miR-19b to suppress its role in inhibiting granulosa cell proliferation. Our study suggested the role of BBOX1-AS1 as a potential target to treat PCOS.

KW - BBOX1-AS1

KW - Granulosa cell proliferation

KW - Polycystic ovary syndrome

KW - miR-19b

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U2 - 10.1186/s12905-023-02632-5

DO - 10.1186/s12905-023-02632-5

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AN - SCOPUS:85171891717

SN - 1472-6874

VL - 23

JO - BMC Women's Health

JF - BMC Women's Health

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ER -

The long non-coding RNA BBOX1 antisense RNA 1 is upregulated in polycystic ovary syndrome (PCOS) and suppresses the role of microRNA-19b in the proliferation of ovarian granulose cells: Short title: BBOX1 antisense RNA 1 in cell proliferation

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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