The modular assembly of clusters is the natural synthetic strategy for the active site of [FeFe] hydrogenase

Ryan D. Bethel; Michael L. Singleton; Marcetta Y. Darensbourg

doi:10.1002/anie.201003747

The modular assembly of clusters is the natural synthetic strategy for the active site of [FeFe] hydrogenase

Ryan D. Bethel, Michael L. Singleton, Marcetta Y. Darensbourg

Chemistry

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The 6Fe supercluster that comprises the [FeFe] hydrogenase active site has been shown to involve a modular buildup of ironsulfur clusters. Only three accessory proteins are needed to produce the CN, CO, and -SCH ₂NHCH₂S- units that bind to the 2Fe2S subcluster precursor. The scaffold/carrier protein inserts the preformed 2Fe subcluster into the apoprotein through a channel, which then closes to encapsulate the completed active site.

Original language	English (US)
Pages (from-to)	8567-8569
Number of pages	3
Journal	Angewandte Chemie - International Edition
Volume	49
Issue number	46
DOIs	https://doi.org/10.1002/anie.201003747
State	Published - Nov 8 2010

Funding

Keywords

active sites
biosynthesis
hydrogenases
ironsulfur clusters

ASJC Scopus subject areas

General Chemistry
Catalysis

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10.1002/anie.201003747

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abstract = "The 6Fe supercluster that comprises the [FeFe] hydrogenase active site has been shown to involve a modular buildup of ironsulfur clusters. Only three accessory proteins are needed to produce the CN, CO, and -SCH 2NHCH2S- units that bind to the 2Fe2S subcluster precursor. The scaffold/carrier protein inserts the preformed 2Fe subcluster into the apoprotein through a channel, which then closes to encapsulate the completed active site.",

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T1 - The modular assembly of clusters is the natural synthetic strategy for the active site of [FeFe] hydrogenase

AU - Bethel, Ryan D.

AU - Singleton, Michael L.

AU - Darensbourg, Marcetta Y.

PY - 2010/11/8

Y1 - 2010/11/8

N2 - The 6Fe supercluster that comprises the [FeFe] hydrogenase active site has been shown to involve a modular buildup of ironsulfur clusters. Only three accessory proteins are needed to produce the CN, CO, and -SCH 2NHCH2S- units that bind to the 2Fe2S subcluster precursor. The scaffold/carrier protein inserts the preformed 2Fe subcluster into the apoprotein through a channel, which then closes to encapsulate the completed active site.

AB - The 6Fe supercluster that comprises the [FeFe] hydrogenase active site has been shown to involve a modular buildup of ironsulfur clusters. Only three accessory proteins are needed to produce the CN, CO, and -SCH 2NHCH2S- units that bind to the 2Fe2S subcluster precursor. The scaffold/carrier protein inserts the preformed 2Fe subcluster into the apoprotein through a channel, which then closes to encapsulate the completed active site.

KW - active sites

KW - biosynthesis

KW - hydrogenases

KW - ironsulfur clusters

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JO - Angewandte Chemie - International Edition

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ER -

The modular assembly of clusters is the natural synthetic strategy for the active site of [FeFe] hydrogenase

Abstract

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Keywords

ASJC Scopus subject areas

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