The most frequent Mycobacterium tuberculosis complex families in Mali (2006-2016) based on spoligotyping

Antieme Combo Georges Togo; Ousmane Kodio; Bassirou Diarra; Moumine Sanogo; Gagni Coulibaly; Sidy Bane; Fatimata Diallo; Anou M. Somboro; Aissata B. Cisse; Bocar Baya; Drissa Goita; Seydou Diabate; Bourahima Kone; Yeya Dit Sadio Sarro; Mamoudou Maiga; Yacouba Toloba; Michael Belson; Susan Orsega; Sounkalo Dao; Robert Leo Murphy; Sophia Siddiqui; Seydou Doumbia; Souleymane Diallo

doi:10.4103/ijmy.ijmy_140_17

The most frequent Mycobacterium tuberculosis complex families in Mali (2006-2016) based on spoligotyping

Antieme Combo Georges Togo, Ousmane Kodio, Bassirou Diarra^*, Moumine Sanogo, Gagni Coulibaly, Sidy Bane, Fatimata Diallo, Anou M. Somboro, Aissata B. Cisse, Bocar Baya, Drissa Goita, Seydou Diabate, Bourahima Kone, Yeya Dit Sadio Sarro, Mamoudou Maiga, Yacouba Toloba, Michael Belson, Susan Orsega, Sounkalo Dao, Robert Leo MurphySophia Siddiqui, Seydou Doumbia, Souleymane Diallo

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

17 Scopus citations

Abstract

Background: To identify strains of Mycobacterium tuberculosis complex (MTBc) circulating in Bamako region during the past 10 years. Methods: From 2006 to 2016, we conducted a cross-sectional study to identify with spoligotyping, clinical isolates from tuberculosis (TB)-infected patients at different stages of their treatments in Bamako, Mali. Results: Among the 904 suspected TB patients included in the study and thereafter tested in our BSL-3 laboratory, 492 (54.4%) had MTBc and therefore underwent spoligotyping. Overall, three subspecies, i.e., MTB T1 (31.9%) and MTB LAM10 (15.3%) from lineage 4 and M. africanum 2 (16.8%) from lineage 6 were the leading causes of TB in Bamako region during the past 10 years. Other spoligotypes such as MTB T3, MTB Haarlem 2, MTB EAI3, and MTB family 33 were also commonly seen from 2010 to 2016. Conclusion: This study showed a high genetic diversity of strains isolated in Bamako region and highlights that M.Tuberculosis T1 strain was the most prevalent. Furthermore, the data indicate an increasing proportion of primary drug resistance overtime in Bamako.

Original language	English (US)
Pages (from-to)	379-386
Number of pages	8
Journal	International Journal of Mycobacteriology
Volume	6
Issue number	4
DOIs	https://doi.org/10.4103/ijmy.ijmy_140_17
State	Published - Oct 1 2017

Funding

This study was partially funded by the USTTB through NIH/R01 grant R01AI110386, the Northwestern University (Chicago, IL, USA) through ACTG U01AI069471. B.D. was supported by a TDR fellowship, TIMS ID B40072, the Special Programme for Research and Training in Tropical Diseases, co-sponsored by the UNICEF, UNDP, World Bank, and WHO.

Keywords

10 years
Bamako
spoligotyping
strain distribution
tuberculosis

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.4103/ijmy.ijmy_140_17

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Togo, A. C. G., Kodio, O., Diarra, B., Sanogo, M., Coulibaly, G., Bane, S., Diallo, F., Somboro, A. M., Cisse, A. B., Baya, B., Goita, D., Diabate, S., Kone, B., Sarro, Y. D. S., Maiga, M., Toloba, Y., Belson, M., Orsega, S., Dao, S., ... Diallo, S. (2017). The most frequent Mycobacterium tuberculosis complex families in Mali (2006-2016) based on spoligotyping. International Journal of Mycobacteriology, 6(4), 379-386. https://doi.org/10.4103/ijmy.ijmy_140_17

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title = "The most frequent Mycobacterium tuberculosis complex families in Mali (2006-2016) based on spoligotyping",

abstract = "Background: To identify strains of Mycobacterium tuberculosis complex (MTBc) circulating in Bamako region during the past 10 years. Methods: From 2006 to 2016, we conducted a cross-sectional study to identify with spoligotyping, clinical isolates from tuberculosis (TB)-infected patients at different stages of their treatments in Bamako, Mali. Results: Among the 904 suspected TB patients included in the study and thereafter tested in our BSL-3 laboratory, 492 (54.4%) had MTBc and therefore underwent spoligotyping. Overall, three subspecies, i.e., MTB T1 (31.9%) and MTB LAM10 (15.3%) from lineage 4 and M. africanum 2 (16.8%) from lineage 6 were the leading causes of TB in Bamako region during the past 10 years. Other spoligotypes such as MTB T3, MTB Haarlem 2, MTB EAI3, and MTB family 33 were also commonly seen from 2010 to 2016. Conclusion: This study showed a high genetic diversity of strains isolated in Bamako region and highlights that M.Tuberculosis T1 strain was the most prevalent. Furthermore, the data indicate an increasing proportion of primary drug resistance overtime in Bamako.",

keywords = "10 years, Bamako, spoligotyping, strain distribution, tuberculosis",

author = "Togo, {Antieme Combo Georges} and Ousmane Kodio and Bassirou Diarra and Moumine Sanogo and Gagni Coulibaly and Sidy Bane and Fatimata Diallo and Somboro, {Anou M.} and Cisse, {Aissata B.} and Bocar Baya and Drissa Goita and Seydou Diabate and Bourahima Kone and Sarro, {Yeya Dit Sadio} and Mamoudou Maiga and Yacouba Toloba and Michael Belson and Susan Orsega and Sounkalo Dao and Murphy, {Robert Leo} and Sophia Siddiqui and Seydou Doumbia and Souleymane Diallo",

note = "Funding Information: This study was partially funded by the USTTB through NIH/R01 grant R01AI110386, the Northwestern University (Chicago, IL, USA) through ACTG U01AI069471. B.D. was supported by a TDR fellowship, TIMS ID B40072, the Special Programme for Research and Training in Tropical Diseases, co-sponsored by the UNICEF, UNDP, World Bank, and WHO. Funding Information: Northwestern University (Chicago, IL, USA) through ACTG U01AI069471",

year = "2017",

month = oct,

day = "1",

doi = "10.4103/ijmy.ijmy_140_17",

language = "English (US)",

volume = "6",

pages = "379--386",

journal = "International Journal of Mycobacteriology",

issn = "2212-5531",

publisher = "Wolters Kluwer Medknow Publications",

number = "4",

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Togo, ACG, Kodio, O, Diarra, B, Sanogo, M, Coulibaly, G, Bane, S, Diallo, F, Somboro, AM, Cisse, AB, Baya, B, Goita, D, Diabate, S, Kone, B, Sarro, YDS, Maiga, M, Toloba, Y, Belson, M, Orsega, S, Dao, S, Murphy, RL, Siddiqui, S, Doumbia, S & Diallo, S 2017, 'The most frequent Mycobacterium tuberculosis complex families in Mali (2006-2016) based on spoligotyping', International Journal of Mycobacteriology, vol. 6, no. 4, pp. 379-386. https://doi.org/10.4103/ijmy.ijmy_140_17

TY - JOUR

T1 - The most frequent Mycobacterium tuberculosis complex families in Mali (2006-2016) based on spoligotyping

AU - Togo, Antieme Combo Georges

AU - Kodio, Ousmane

AU - Diarra, Bassirou

AU - Sanogo, Moumine

AU - Coulibaly, Gagni

AU - Bane, Sidy

AU - Diallo, Fatimata

AU - Somboro, Anou M.

AU - Cisse, Aissata B.

AU - Baya, Bocar

AU - Goita, Drissa

AU - Diabate, Seydou

AU - Kone, Bourahima

AU - Sarro, Yeya Dit Sadio

AU - Maiga, Mamoudou

AU - Toloba, Yacouba

AU - Belson, Michael

AU - Orsega, Susan

AU - Dao, Sounkalo

AU - Murphy, Robert Leo

AU - Siddiqui, Sophia

AU - Doumbia, Seydou

AU - Diallo, Souleymane

N1 - Funding Information: This study was partially funded by the USTTB through NIH/R01 grant R01AI110386, the Northwestern University (Chicago, IL, USA) through ACTG U01AI069471. B.D. was supported by a TDR fellowship, TIMS ID B40072, the Special Programme for Research and Training in Tropical Diseases, co-sponsored by the UNICEF, UNDP, World Bank, and WHO. Funding Information: Northwestern University (Chicago, IL, USA) through ACTG U01AI069471

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Background: To identify strains of Mycobacterium tuberculosis complex (MTBc) circulating in Bamako region during the past 10 years. Methods: From 2006 to 2016, we conducted a cross-sectional study to identify with spoligotyping, clinical isolates from tuberculosis (TB)-infected patients at different stages of their treatments in Bamako, Mali. Results: Among the 904 suspected TB patients included in the study and thereafter tested in our BSL-3 laboratory, 492 (54.4%) had MTBc and therefore underwent spoligotyping. Overall, three subspecies, i.e., MTB T1 (31.9%) and MTB LAM10 (15.3%) from lineage 4 and M. africanum 2 (16.8%) from lineage 6 were the leading causes of TB in Bamako region during the past 10 years. Other spoligotypes such as MTB T3, MTB Haarlem 2, MTB EAI3, and MTB family 33 were also commonly seen from 2010 to 2016. Conclusion: This study showed a high genetic diversity of strains isolated in Bamako region and highlights that M.Tuberculosis T1 strain was the most prevalent. Furthermore, the data indicate an increasing proportion of primary drug resistance overtime in Bamako.

AB - Background: To identify strains of Mycobacterium tuberculosis complex (MTBc) circulating in Bamako region during the past 10 years. Methods: From 2006 to 2016, we conducted a cross-sectional study to identify with spoligotyping, clinical isolates from tuberculosis (TB)-infected patients at different stages of their treatments in Bamako, Mali. Results: Among the 904 suspected TB patients included in the study and thereafter tested in our BSL-3 laboratory, 492 (54.4%) had MTBc and therefore underwent spoligotyping. Overall, three subspecies, i.e., MTB T1 (31.9%) and MTB LAM10 (15.3%) from lineage 4 and M. africanum 2 (16.8%) from lineage 6 were the leading causes of TB in Bamako region during the past 10 years. Other spoligotypes such as MTB T3, MTB Haarlem 2, MTB EAI3, and MTB family 33 were also commonly seen from 2010 to 2016. Conclusion: This study showed a high genetic diversity of strains isolated in Bamako region and highlights that M.Tuberculosis T1 strain was the most prevalent. Furthermore, the data indicate an increasing proportion of primary drug resistance overtime in Bamako.

KW - 10 years

KW - Bamako

KW - spoligotyping

KW - strain distribution

KW - tuberculosis

UR - http://www.scopus.com/inward/record.url?scp=85034984624&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85034984624&partnerID=8YFLogxK

U2 - 10.4103/ijmy.ijmy_140_17

DO - 10.4103/ijmy.ijmy_140_17

M3 - Review article

C2 - 29171452

AN - SCOPUS:85034984624

SN - 2212-5531

VL - 6

SP - 379

EP - 386

JO - International Journal of Mycobacteriology

JF - International Journal of Mycobacteriology

IS - 4

ER -

The most frequent Mycobacterium tuberculosis complex families in Mali (2006-2016) based on spoligotyping

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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