The mouse Clock locus: Sequence and comparative analysis of 204 kb from mouse chromosome 5

Lisa D Wilsbacher, A. M. Sangoram, M. P. Antoch, J. S. Takahashi*

*Corresponding author for this work

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The Clock gene encodes a basic helix-loop-helix (bHLH)-PAS transcription factor that regulates circadian rhythms in mice. We previously cloned Clock in mouse and human using a battery of behavioral and molecular techniques, including shotgun sequencing of two bacterial artificial chromosome (BAC) clones. Here we report the finished sequence of a 204-kb region from mouse chromosome 5. This region contains the complete loci for the Clock and Tpardl (pFT27) genes, as well as the 3′ partial locus of the Neuromedin U gene; sequence analysis also suggests the presence of two previously unidentified genes. In addition, we provide a comparative genomic sequence analysis with the syntenic region from human chromosome 4. Finally, a new BAC transgenic line indicates that the genomic region that is sufficient for rescue of the Clock mutant phenotype is no greater than 120 kb and tightly flanks the 3′ end of the Clock gene.

Original languageEnglish (US)
Pages (from-to)1928-1940
Number of pages13
JournalGenome Research
Volume10
Issue number12
DOIs
StatePublished - Dec 1 2000

Fingerprint

Chromosomes, Human, Pair 5
Sequence Analysis
Bacterial Artificial Chromosomes
Genes
Basic Helix-Loop-Helix Transcription Factors
Chromosomes, Human, Pair 4
Firearms
Human Chromosomes
Circadian Rhythm
Clone Cells
Phenotype

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Wilsbacher, Lisa D ; Sangoram, A. M. ; Antoch, M. P. ; Takahashi, J. S. / The mouse Clock locus : Sequence and comparative analysis of 204 kb from mouse chromosome 5. In: Genome Research. 2000 ; Vol. 10, No. 12. pp. 1928-1940.
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The mouse Clock locus : Sequence and comparative analysis of 204 kb from mouse chromosome 5. / Wilsbacher, Lisa D; Sangoram, A. M.; Antoch, M. P.; Takahashi, J. S.

In: Genome Research, Vol. 10, No. 12, 01.12.2000, p. 1928-1940.

Research output: Contribution to journalArticle

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