The mouse metallothionein-I gene is transcriptionally regulated by cadmium following transfection into human or mouse cells

Kelly E. Mayo*, Raphael Warren, Richard D. Palmiter

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

154 Scopus citations

Abstract

Recombinant vectors containing the mouse metallothionein-I gene (MT-I) and the Escherichia coli xanthine-guanine phosphoribosyltransferase gene (gpt) were used to transfect human hgprt- HeLa cells. Transfected MT-I genes are transcriptionally regulated by cadmium but not by glucocorticoids. S1 mapping indicates that the transcripts from transfected MT-I genes begin at the correct transcription initiation site. We also transfected mouse tk- L cells with a vector containing the mouse MT-I gene and the herpes simplex virus-I thymidine kinase gene. MT-I gene transcription is regulated by cadmium but not by glucocorticoids in this homologous system as well. Finally, we fused the MT-I gene promoter/regulatory region to the thymidine kinase structural gene. Thymidine kinase activity is regulated by cadmium when this fusion gene is transfected into mouse tk- L cells. Deletion mapping experiments indicate that the DNA sequences necessary for regulation of the MT-I gene by cadmium lie within 148 bp of its transcription start site.

Original languageEnglish (US)
Pages (from-to)99-108
Number of pages10
JournalCell
Volume29
Issue number1
DOIs
StatePublished - May 1982

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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