The murine CD99-related molecule CD99-like 2 (CD99L2) is an adhesion molecule involved in the inflammatory response

Alan R. Schenkel, Eric M. Dufour, Tina W. Chew, Emily Sorg, William A. Muller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

CD99, a glycoprotein found on the surfaces of leukocytes and concentrated at the borders of endothelial cells, plays a major role in the migration of leukocytes across endothelial cells into sites of inflammation, and has other roles in thymocyte development. The human and mouse genomes encode only two proteins related to CD99. One of these, XGA, is a red blood cell surface antigen. The function of the other, CD99-like 2 (CD99L2), is not known. We cloned mouse CD99L2 and used CD99L2 isolated from transfected cells to raise specific antibodies. Similar to human CD99, CD99L2 was expressed at the borders between transfected cells as well as on mouse leukocytes and vascular endothelial cells in situ. Transfection of L cell fibroblasts with CD99L2 imparted to them the ability to adhere to each other in a divalent cation-dependent, homophilic manner. Anti-CD99L2 antibody blocked influx of neutrophils and monocytes into a site of inflammation in vivo.

Original languageEnglish (US)
Pages (from-to)227-237
Number of pages11
JournalCell Communication and Adhesion
Volume14
Issue number5
DOIs
StatePublished - Sep 2007

Keywords

  • Adhesion molecule
  • CD99
  • CD99L2
  • Endothelium
  • Inflammation
  • Leukocyte
  • MIC2 surface antigen

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

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