Abstract
CD99, a glycoprotein found on the surfaces of leukocytes and concentrated at the borders of endothelial cells, plays a major role in the migration of leukocytes across endothelial cells into sites of inflammation, and has other roles in thymocyte development. The human and mouse genomes encode only two proteins related to CD99. One of these, XGA, is a red blood cell surface antigen. The function of the other, CD99-like 2 (CD99L2), is not known. We cloned mouse CD99L2 and used CD99L2 isolated from transfected cells to raise specific antibodies. Similar to human CD99, CD99L2 was expressed at the borders between transfected cells as well as on mouse leukocytes and vascular endothelial cells in situ. Transfection of L cell fibroblasts with CD99L2 imparted to them the ability to adhere to each other in a divalent cation-dependent, homophilic manner. Anti-CD99L2 antibody blocked influx of neutrophils and monocytes into a site of inflammation in vivo.
Original language | English (US) |
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Pages (from-to) | 227-237 |
Number of pages | 11 |
Journal | Cell Communication and Adhesion |
Volume | 14 |
Issue number | 5 |
DOIs | |
State | Published - Sep 2007 |
Funding
Received 11 May 2007; accepted 13 August 2007. We wish to thank Drs. Cheryl Guyre and Johanne Kaplan for help constructing the murine CD99L2-Fc plasmid and Ronald M. Liebman for expert technical assistance. This study was funded by a sponsored research grant from Genzyme Corporation and NIH grants HL064774 and HL046849 to W.A.M. Address correspondence to William A. Muller, MD, PhD, Department of Pathology Northwestern University, Feinberg School of Medicine, Ward Building 6-204, 303 East Chicago Avenue, Chicago, IL 60611-3008. Phone: 312-503-8144, Fax: 312-503-8249. E-mail: [email protected]
Keywords
- Adhesion molecule
- CD99
- CD99L2
- Endothelium
- Inflammation
- Leukocyte
- MIC2 surface antigen
ASJC Scopus subject areas
- Clinical Biochemistry
- Cell Biology