The Nasopharyngeal Microbiota of Children with Respiratory Infections in Botswana

Matthew S. Kelly*, Michael G. Surette, Marek Smieja, Jeffrey M. Pernica, Laura Rossi, Kathy Luinstra, Andrew P. Steenhoff, Kristen A. Feemster, David M. Goldfarb, Tonya Arscott-Mills, Sefelani Boiditswe, Ikanyeng Rulaganyang, Charles Muthoga, Letang Gaofiwe, Tiny Mazhani, John F. Rawls, Coleen K. Cunningham, Samir S. Shah, Patrick C. Seed

*Corresponding author for this work

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Background: Nearly half of child pneumonia deaths occur in sub-Saharan Africa. Microbial communities in the nasopharynx are a reservoir for pneumonia pathogens and remain poorly described in African children. Methods: Nasopharyngeal swabs were collected from children with pneumonia (N = 204), children with upper respiratory infection symptoms (N = 55) and healthy children (N = 60) in Botswana between April 2012 and April 2014. We sequenced the V3 region of the bacterial 16S ribosomal RNA gene and used partitioning around medoids to cluster samples into microbiota biotypes. We then used multivariable logistic regression to examine whether microbiota biotypes were associated with pneumonia and upper respiratory infection symptoms. Results: Mean ages of children with pneumonia, children with upper respiratory infection symptoms and healthy children were 8.2, 11.4 and 8.0 months, respectively. Clustering of nasopharyngeal microbiota identified 5 distinct biotypes: Corynebacterium/Dolosigranulum-dominant (23%), Haemophilus-dominant (11%), Moraxella-dominant (24%), Staphylococcus-dominant (13%) and Streptococcus-dominant (28%). The Haemophilus-dominant [odds ratio (OR): 13.55; 95% confidence interval (CI): 2.10-87.26], the Staphylococcus-dominant (OR: 8.27; 95% CI: 2.13-32.14) and the Streptococcus-dominant (OR: 39.97; 95% CI: 6.63-241.00) biotypes were associated with pneumonia. The Moraxella-dominant (OR: 3.71; 95% CI: 1.09-12.64) and Streptococcus-dominant (OR: 12.26; 95% CI: 1.81-83.06) biotypes were associated with upper respiratory infection symptoms. In children with pneumonia, HIV infection was associated with a lower relative abundance of Dolosigranulum (P = 0.03). Conclusions: Pneumonia and upper respiratory infection symptoms are associated with distinct nasopharyngeal microbiota biotypes in African children. A lower abundance of the commensal genus Dolosigranulum may contribute to the higher pneumonia risk of HIV-infected children.

Original languageEnglish (US)
Pages (from-to)e211-e218
JournalPediatric Infectious Disease Journal
Volume36
Issue number9
DOIs
StatePublished - Sep 1 2017

Keywords

  • children
  • microbial communities
  • microbiota
  • pneumonia
  • respiratory infections

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Microbiology (medical)
  • Infectious Diseases

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    Kelly, M. S., Surette, M. G., Smieja, M., Pernica, J. M., Rossi, L., Luinstra, K., Steenhoff, A. P., Feemster, K. A., Goldfarb, D. M., Arscott-Mills, T., Boiditswe, S., Rulaganyang, I., Muthoga, C., Gaofiwe, L., Mazhani, T., Rawls, J. F., Cunningham, C. K., Shah, S. S., & Seed, P. C. (2017). The Nasopharyngeal Microbiota of Children with Respiratory Infections in Botswana. Pediatric Infectious Disease Journal, 36(9), e211-e218. https://doi.org/10.1097/INF.0000000000001607