Abstract
Nematode degenerins have been implicated in touch sensitivity and other forms of mechanosensation. Certain mutations in several degenerin genes cause the swelling, vacuolation, and death of neurons, and other mutations in the muscle degenerin gene unc-105 cause hypercontraction. Here, we confirm that unc-105 encodes an ion channel and show that it is constitutively active when mutated. These mutations disrupt different regions of the channel and have different effects on its gating. The UNC-105 channels are permeable to small monovalent cations but show voltage-dependent block by Ca2+ and Mg2+. Amiloride also produces voltage-dependent block, consistent with a single binding site 65% into the electric field. Mammalian cells expressing the mutant channels accumulate membranous whorls and multicompartment vacuoles, hallmarks of degenerin-induced cell death across species.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1231-1241 |
| Number of pages | 11 |
| Journal | Neuron |
| Volume | 20 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 1998 |
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ASJC Scopus subject areas
- Neuroscience(all)
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The nematode degenerin UNC-105 forms ion channels that are activated by degeneration- or hypercontraction-causing mutations. / García-Añoveros, Jaime; García, Jesus A.; Liu, Jing Dong; Corey, David P.
In: Neuron, Vol. 20, No. 6, 06.1998, p. 1231-1241.Research output: Contribution to journal › Article
TY - JOUR
T1 - The nematode degenerin UNC-105 forms ion channels that are activated by degeneration- or hypercontraction-causing mutations
AU - García-Añoveros, Jaime
AU - García, Jesus A.
AU - Liu, Jing Dong
AU - Corey, David P.
PY - 1998/6
Y1 - 1998/6
N2 - Nematode degenerins have been implicated in touch sensitivity and other forms of mechanosensation. Certain mutations in several degenerin genes cause the swelling, vacuolation, and death of neurons, and other mutations in the muscle degenerin gene unc-105 cause hypercontraction. Here, we confirm that unc-105 encodes an ion channel and show that it is constitutively active when mutated. These mutations disrupt different regions of the channel and have different effects on its gating. The UNC-105 channels are permeable to small monovalent cations but show voltage-dependent block by Ca2+ and Mg2+. Amiloride also produces voltage-dependent block, consistent with a single binding site 65% into the electric field. Mammalian cells expressing the mutant channels accumulate membranous whorls and multicompartment vacuoles, hallmarks of degenerin-induced cell death across species.
AB - Nematode degenerins have been implicated in touch sensitivity and other forms of mechanosensation. Certain mutations in several degenerin genes cause the swelling, vacuolation, and death of neurons, and other mutations in the muscle degenerin gene unc-105 cause hypercontraction. Here, we confirm that unc-105 encodes an ion channel and show that it is constitutively active when mutated. These mutations disrupt different regions of the channel and have different effects on its gating. The UNC-105 channels are permeable to small monovalent cations but show voltage-dependent block by Ca2+ and Mg2+. Amiloride also produces voltage-dependent block, consistent with a single binding site 65% into the electric field. Mammalian cells expressing the mutant channels accumulate membranous whorls and multicompartment vacuoles, hallmarks of degenerin-induced cell death across species.
UR - http://www.scopus.com/inward/record.url?scp=0032102486&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032102486&partnerID=8YFLogxK
U2 - 10.1016/S0896-6273(00)80503-6
DO - 10.1016/S0896-6273(00)80503-6
M3 - Article
C2 - 9655510
AN - SCOPUS:0032102486
VL - 20
SP - 1231
EP - 1241
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 6
ER -