The nematode degenerin UNC-105 forms ion channels that are activated by degeneration- or hypercontraction-causing mutations

Jaime García-Añoveros, Jesus A. García, Jing Dong Liu, David P. Corey*

*Corresponding author for this work

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Nematode degenerins have been implicated in touch sensitivity and other forms of mechanosensation. Certain mutations in several degenerin genes cause the swelling, vacuolation, and death of neurons, and other mutations in the muscle degenerin gene unc-105 cause hypercontraction. Here, we confirm that unc-105 encodes an ion channel and show that it is constitutively active when mutated. These mutations disrupt different regions of the channel and have different effects on its gating. The UNC-105 channels are permeable to small monovalent cations but show voltage-dependent block by Ca2+ and Mg2+. Amiloride also produces voltage-dependent block, consistent with a single binding site 65% into the electric field. Mammalian cells expressing the mutant channels accumulate membranous whorls and multicompartment vacuoles, hallmarks of degenerin-induced cell death across species.

Original languageEnglish (US)
Pages (from-to)1231-1241
Number of pages11
JournalNeuron
Volume20
Issue number6
DOIs
StatePublished - Jan 1 1998

Fingerprint

Degenerin Sodium Channels
Ion Channels
Mutation
Monovalent Cations
Amiloride
Touch
Vacuoles
Genes
Cell Death
Binding Sites
Neurons
Muscles

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

García-Añoveros, Jaime ; García, Jesus A. ; Liu, Jing Dong ; Corey, David P. / The nematode degenerin UNC-105 forms ion channels that are activated by degeneration- or hypercontraction-causing mutations. In: Neuron. 1998 ; Vol. 20, No. 6. pp. 1231-1241.
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abstract = "Nematode degenerins have been implicated in touch sensitivity and other forms of mechanosensation. Certain mutations in several degenerin genes cause the swelling, vacuolation, and death of neurons, and other mutations in the muscle degenerin gene unc-105 cause hypercontraction. Here, we confirm that unc-105 encodes an ion channel and show that it is constitutively active when mutated. These mutations disrupt different regions of the channel and have different effects on its gating. The UNC-105 channels are permeable to small monovalent cations but show voltage-dependent block by Ca2+ and Mg2+. Amiloride also produces voltage-dependent block, consistent with a single binding site 65{\%} into the electric field. Mammalian cells expressing the mutant channels accumulate membranous whorls and multicompartment vacuoles, hallmarks of degenerin-induced cell death across species.",
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The nematode degenerin UNC-105 forms ion channels that are activated by degeneration- or hypercontraction-causing mutations. / García-Añoveros, Jaime; García, Jesus A.; Liu, Jing Dong; Corey, David P.

In: Neuron, Vol. 20, No. 6, 01.01.1998, p. 1231-1241.

Research output: Contribution to journalArticle

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AB - Nematode degenerins have been implicated in touch sensitivity and other forms of mechanosensation. Certain mutations in several degenerin genes cause the swelling, vacuolation, and death of neurons, and other mutations in the muscle degenerin gene unc-105 cause hypercontraction. Here, we confirm that unc-105 encodes an ion channel and show that it is constitutively active when mutated. These mutations disrupt different regions of the channel and have different effects on its gating. The UNC-105 channels are permeable to small monovalent cations but show voltage-dependent block by Ca2+ and Mg2+. Amiloride also produces voltage-dependent block, consistent with a single binding site 65% into the electric field. Mammalian cells expressing the mutant channels accumulate membranous whorls and multicompartment vacuoles, hallmarks of degenerin-induced cell death across species.

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