The neuroprotective marine compound psammaplysene A binds the RNA-binding protein HNRNPK

Marco Boccitto, Nayoung Lee, Satoshi Sakamoto, Lynn A. Spruce, Hiroshi Handa, Jon Clardy, Steven H. Seeholzer, Robert G. Kalb*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

In previous work, we characterized the strong neuroprotective properties of the marine compound Psammaplysene A (PA) in in vitro and in vivo models of neurodegeneration. Based on its strong neuroprotective activity, the current work attempts to identify the physical target of PA to gain mechanistic insight into its molecular action. Two distinct methods, used in parallel, to purify protein-binding partners of PA led to the identification of HNRNPK as a direct target of PA. Based on surface plasmon resonance, we find that the binding of PA to HNRNPK is RNA-dependent. These findings suggest a role for HNRNPK-dependent processes in neurodegeneration/neuroprotection, and warrant further study of HNRNPK in this context.

Original languageEnglish (US)
Article number246
JournalMarine Drugs
Volume15
Issue number8
DOIs
StatePublished - Aug 2017

Keywords

  • Foxo
  • HNRNPK
  • Neurodegeneration
  • Psammaplysene A
  • RNA metabolism

ASJC Scopus subject areas

  • Drug Discovery

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