The novel gene twenty-four defines a critical translational step in the Drosophila clock

Chunghun Lim, Jongbin Lee, Changtaek Choi, Valerie Kilman, Juwon Kim, Sung Mi Park, Sung Key Jang, Ravi Allada*, Joonho Choe

*Corresponding author for this work

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Daily oscillations of gene expression underlie circadian behaviours in multicellular organisms. While attention has been focused on transcriptional and post-translational mechanisms, other post-transcriptional modes have been less clearly delineated. Here we report mutants of a novel Drosophila gene twenty-four (tyf) that show weak behavioural rhythms. Weak rhythms are accompanied by marked reductions in the levels of the clock protein Period (PER) as well as more modest effects on Timeless (TIM). Nonetheless, PER induction in pacemaker neurons can rescue tyf mutant rhythms. TYF associates with a 5′-cap-binding complex, poly(A)-binding protein (PABP), as well as per and tim transcripts. Furthermore, TYF activates reporter expression when tethered to reporter messenger RNA even in vitro. Taken together, these data indicate that TYF potently activates PER translation in pacemaker neurons to sustain robust rhythms, revealing a new and important role for translational control in the Drosophila circadian clock.

Original languageEnglish (US)
Pages (from-to)399-403
Number of pages5
JournalNature
Volume470
Issue number7334
DOIs
StatePublished - Feb 17 2011

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Cite this

Lim, C., Lee, J., Choi, C., Kilman, V., Kim, J., Park, S. M., Jang, S. K., Allada, R., & Choe, J. (2011). The novel gene twenty-four defines a critical translational step in the Drosophila clock. Nature, 470(7334), 399-403. https://doi.org/10.1038/nature09728