The nucleotide sequence of a full length cDNA clone encoding rat liver urate oxidase

Keith Alvares; Mohan R. Nemali; P. Gopal Reddy; Xuedong Wang; M. Sambasiva Rao; Janardan K. Reddy

doi:10.1016/0006-291X(89)92820-9

The nucleotide sequence of a full length cDNA clone encoding rat liver urate oxidase

Keith Alvares^*, Mohan R. Nemali, P. Gopal Reddy, Xuedong Wang, M. Sambasiva Rao, Janardan K. Reddy

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Recently we reported the sequence of a cDNA clone (pUOX-1), isolated from a λgtll cDNA library, which encoded for rat liver urate oxidase (EC 1.7.3.3), but this clone lacked the nucleotide sequences encoding the N-terminal region for this enzyme. Using the cDNA insert from the pUOX-1 clone as a probe, we have now isolated a full length cDNA clone, pUOX-2, from a λgt10 library by plaque hybridization. Nucleotide sequence analysis of the pUOX-2 clone showed that it has 1379 base pairs with an open reading frame coding for 303 amino acid residues corresponding to a molecular mass of 34,931 daltons. In addition to the open reading frame the pUOX-2 contains 439 bp of 3′-untranslated and 41 bp of 5′-untranslated sequences. The consensus polyadenylation signal AATAAA precedes a stretch of poly(A)+ residues at the 3′ end.

Original language	English (US)
Pages (from-to)	991-995
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	158
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(89)92820-9
State	Published - Feb 15 1989

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "The nucleotide sequence of a full length cDNA clone encoding rat liver urate oxidase",

abstract = "Recently we reported the sequence of a cDNA clone (pUOX-1), isolated from a λgtll cDNA library, which encoded for rat liver urate oxidase (EC 1.7.3.3), but this clone lacked the nucleotide sequences encoding the N-terminal region for this enzyme. Using the cDNA insert from the pUOX-1 clone as a probe, we have now isolated a full length cDNA clone, pUOX-2, from a λgt10 library by plaque hybridization. Nucleotide sequence analysis of the pUOX-2 clone showed that it has 1379 base pairs with an open reading frame coding for 303 amino acid residues corresponding to a molecular mass of 34,931 daltons. In addition to the open reading frame the pUOX-2 contains 439 bp of 3′-untranslated and 41 bp of 5′-untranslated sequences. The consensus polyadenylation signal AATAAA precedes a stretch of poly(A)+ residues at the 3′ end.",

author = "Keith Alvares and Nemali, {Mohan R.} and Reddy, {P. Gopal} and Xuedong Wang and Rao, {M. Sambasiva} and Reddy, {Janardan K.}",

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doi = "10.1016/0006-291X(89)92820-9",

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AU - Alvares, Keith

AU - Nemali, Mohan R.

AU - Reddy, P. Gopal

AU - Wang, Xuedong

AU - Rao, M. Sambasiva

AU - Reddy, Janardan K.

N1 - Funding Information: was supported by U.S. Public Kathleen Stenson for typin,g

PY - 1989/2/15

Y1 - 1989/2/15

N2 - Recently we reported the sequence of a cDNA clone (pUOX-1), isolated from a λgtll cDNA library, which encoded for rat liver urate oxidase (EC 1.7.3.3), but this clone lacked the nucleotide sequences encoding the N-terminal region for this enzyme. Using the cDNA insert from the pUOX-1 clone as a probe, we have now isolated a full length cDNA clone, pUOX-2, from a λgt10 library by plaque hybridization. Nucleotide sequence analysis of the pUOX-2 clone showed that it has 1379 base pairs with an open reading frame coding for 303 amino acid residues corresponding to a molecular mass of 34,931 daltons. In addition to the open reading frame the pUOX-2 contains 439 bp of 3′-untranslated and 41 bp of 5′-untranslated sequences. The consensus polyadenylation signal AATAAA precedes a stretch of poly(A)+ residues at the 3′ end.

AB - Recently we reported the sequence of a cDNA clone (pUOX-1), isolated from a λgtll cDNA library, which encoded for rat liver urate oxidase (EC 1.7.3.3), but this clone lacked the nucleotide sequences encoding the N-terminal region for this enzyme. Using the cDNA insert from the pUOX-1 clone as a probe, we have now isolated a full length cDNA clone, pUOX-2, from a λgt10 library by plaque hybridization. Nucleotide sequence analysis of the pUOX-2 clone showed that it has 1379 base pairs with an open reading frame coding for 303 amino acid residues corresponding to a molecular mass of 34,931 daltons. In addition to the open reading frame the pUOX-2 contains 439 bp of 3′-untranslated and 41 bp of 5′-untranslated sequences. The consensus polyadenylation signal AATAAA precedes a stretch of poly(A)+ residues at the 3′ end.

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JO - Biochemical and Biophysical Research Communications

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The nucleotide sequence of a full length cDNA clone encoding rat liver urate oxidase

Abstract

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