The nucleotide sequence of a full length cDNA clone encoding rat liver urate oxidase

Keith Alvares*, Mohan R. Nemali, P. Gopal Reddy, Xuedong Wang, Sambasiva Rao Musunuri, Janardan K Reddy

*Corresponding author for this work

Research output: Contribution to journalArticle

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Abstract

Recently we reported the sequence of a cDNA clone (pUOX-1), isolated from a λgtll cDNA library, which encoded for rat liver urate oxidase (EC 1.7.3.3), but this clone lacked the nucleotide sequences encoding the N-terminal region for this enzyme. Using the cDNA insert from the pUOX-1 clone as a probe, we have now isolated a full length cDNA clone, pUOX-2, from a λgt10 library by plaque hybridization. Nucleotide sequence analysis of the pUOX-2 clone showed that it has 1379 base pairs with an open reading frame coding for 303 amino acid residues corresponding to a molecular mass of 34,931 daltons. In addition to the open reading frame the pUOX-2 contains 439 bp of 3′-untranslated and 41 bp of 5′-untranslated sequences. The consensus polyadenylation signal AATAAA precedes a stretch of poly(A)+ residues at the 3′ end.

Original languageEnglish (US)
Pages (from-to)991-995
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume158
Issue number3
DOIs
Publication statusPublished - Feb 15 1989

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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