The oocyte lamin persists as a single major component of the nuclear lamina during embryonic development of the surf clam

G. Dessev, Robert Goldman

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Nuclei and nuclear lamina-enriched fractions, isolated from 1 to 5-day-old embryos of the surf clam, Spisula solidissima, contain only one major lamin protein, which appears to be identical to the oocyte lamin (L67), as judged by 2D IEF/SDS PAGE, reactivity with a polyclonal antibody directed against L67 and 125I tryptic peptide mapping. The same protein is also present in liver, muscle, nerve and testis from adult animals. No proteins - recognized by several poly- and monoclonal antibodies, specific for somatic lamins from different vertebrate species or the oocyte lamin L(III) of Xenopus - have been detected in nuclei or NL-enriched preparations, isolated from embryos or adult tissues. Synthesis of L67 is detectable in embryos 2h after fertilization; it reaches a maximum in 6h-old embryos and gradually declines thereafter. These results argue that the composition of the NL bears no obvious relationship to the structural and functional changes that take place during the embryonic development of this invertebrate.

Original languageEnglish (US)
Pages (from-to)267-274
Number of pages8
JournalInternational Journal of Developmental Biology
Volume34
Issue number2
StatePublished - Oct 15 1990

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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