The Pediatric Obesity Microbiome and Metabolism Study (POMMS): Methods, Baseline Data, and Early Insights

Jessica R. McCann; Nathan A. Bihlmeyer; Kimberly Roche; Cameron Catherine; Jayanth Jawahar; Lydia Coulter Kwee; Noelle E. Younge; Justin Silverman; Olga Ilkayeva; Charles Sarria; Alexandra Zizzi; Janet Wootton; Lisa Poppe; Paul Anderson; Michelle Arlotto; Zhengzheng Wei; Joshua A. Granek; Raphael H. Valdivia; Lawrence A. David; Holly K. Dressman; Christopher B. Newgard; Svati H. Shah; Patrick C. Seed; John F. Rawls; Sarah C. Armstrong

doi:10.1002/oby.23081

The Pediatric Obesity Microbiome and Metabolism Study (POMMS): Methods, Baseline Data, and Early Insights

Jessica R. McCann, Nathan A. Bihlmeyer, Kimberly Roche, Cameron Catherine, Jayanth Jawahar, Lydia Coulter Kwee, Noelle E. Younge, Justin Silverman, Olga Ilkayeva, Charles Sarria, Alexandra Zizzi, Janet Wootton, Lisa Poppe, Paul Anderson, Michelle Arlotto, Zhengzheng Wei, Joshua A. Granek, Raphael H. Valdivia, Lawrence A. David, Holly K. DressmanChristopher B. Newgard, Svati H. Shah, Patrick C. Seed^*, John F. Rawls^*, Sarah C. Armstrong^*

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Objective: The purpose of this study was to establish a biorepository of clinical, metabolomic, and microbiome samples from adolescents with obesity as they undergo lifestyle modification. Methods: A total of 223 adolescents aged 10 to 18 years with BMI ≥95th percentile were enrolled, along with 71 healthy weight participants. Clinical data, fasting serum, and fecal samples were collected at repeated intervals over 6 months. Herein, the study design, data collection methods, and interim analysis—including targeted serum metabolite measurements and fecal 16S ribosomal RNA gene amplicon sequencing among adolescents with obesity (n = 27) and healthy weight controls (n = 27)—are presented. Results: Adolescents with obesity have higher serum alanine aminotransferase, C-reactive protein, and glycated hemoglobin, and they have lower high-density lipoprotein cholesterol when compared with healthy weight controls. Metabolomics revealed differences in branched-chain amino acid–related metabolites. Also observed was a differential abundance of specific microbial taxa and lower species diversity among adolescents with obesity when compared with the healthy weight group. Conclusions: The Pediatric Metabolism and Microbiome Study (POMMS) biorepository is available as a shared resource. Early findings suggest evidence of a metabolic signature of obesity unique to adolescents, along with confirmation of previously reported findings that describe metabolic and microbiome markers of obesity.

Original language	English (US)
Pages (from-to)	569-578
Number of pages	10
Journal	Obesity
Volume	29
Issue number	3
DOIs	https://doi.org/10.1002/oby.23081
State	Published - Mar 2021

Funding

We would like to thank the study participants and their families for their involvement in this study. All raw sequencing data are available via the NIH Sequence Read Archive (SRA) (submission number PRJNA647465). Information on how to request access to the clinical sample biorepository and deidentified participant data can be found at https://sites.duke.edu/pomms This study was supported by the NIH (R24 DK110492) and the American Heart Association (7SFRN33670990).

ASJC Scopus subject areas

Medicine (miscellaneous)
Endocrinology, Diabetes and Metabolism
Endocrinology
Nutrition and Dietetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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McCann, J. R., Bihlmeyer, N. A., Roche, K., Catherine, C., Jawahar, J., Kwee, L. C., Younge, N. E., Silverman, J., Ilkayeva, O., Sarria, C., Zizzi, A., Wootton, J., Poppe, L., Anderson, P., Arlotto, M., Wei, Z., Granek, J. A., Valdivia, R. H., David, L. A., ... Armstrong, S. C. (2021). The Pediatric Obesity Microbiome and Metabolism Study (POMMS): Methods, Baseline Data, and Early Insights. Obesity, 29(3), 569-578. https://doi.org/10.1002/oby.23081

@article{6a81522f68c742c5ad91555ccb643713,

title = "The Pediatric Obesity Microbiome and Metabolism Study (POMMS): Methods, Baseline Data, and Early Insights",

abstract = "Objective: The purpose of this study was to establish a biorepository of clinical, metabolomic, and microbiome samples from adolescents with obesity as they undergo lifestyle modification. Methods: A total of 223 adolescents aged 10 to 18 years with BMI ≥95th percentile were enrolled, along with 71 healthy weight participants. Clinical data, fasting serum, and fecal samples were collected at repeated intervals over 6 months. Herein, the study design, data collection methods, and interim analysis—including targeted serum metabolite measurements and fecal 16S ribosomal RNA gene amplicon sequencing among adolescents with obesity (n = 27) and healthy weight controls (n = 27)—are presented. Results: Adolescents with obesity have higher serum alanine aminotransferase, C-reactive protein, and glycated hemoglobin, and they have lower high-density lipoprotein cholesterol when compared with healthy weight controls. Metabolomics revealed differences in branched-chain amino acid–related metabolites. Also observed was a differential abundance of specific microbial taxa and lower species diversity among adolescents with obesity when compared with the healthy weight group. Conclusions: The Pediatric Metabolism and Microbiome Study (POMMS) biorepository is available as a shared resource. Early findings suggest evidence of a metabolic signature of obesity unique to adolescents, along with confirmation of previously reported findings that describe metabolic and microbiome markers of obesity.",

author = "McCann, {Jessica R.} and Bihlmeyer, {Nathan A.} and Kimberly Roche and Cameron Catherine and Jayanth Jawahar and Kwee, {Lydia Coulter} and Younge, {Noelle E.} and Justin Silverman and Olga Ilkayeva and Charles Sarria and Alexandra Zizzi and Janet Wootton and Lisa Poppe and Paul Anderson and Michelle Arlotto and Zhengzheng Wei and Granek, {Joshua A.} and Valdivia, {Raphael H.} and David, {Lawrence A.} and Dressman, {Holly K.} and Newgard, {Christopher B.} and Shah, {Svati H.} and Seed, {Patrick C.} and Rawls, {John F.} and Armstrong, {Sarah C.}",

note = "Publisher Copyright: {\textcopyright} 2021 The Obesity Society.",

year = "2021",

month = mar,

doi = "10.1002/oby.23081",

language = "English (US)",

volume = "29",

pages = "569--578",

journal = "Obesity",

issn = "1930-7381",

publisher = "John Wiley and Sons Inc.",

number = "3",

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McCann, JR, Bihlmeyer, NA, Roche, K, Catherine, C, Jawahar, J, Kwee, LC, Younge, NE, Silverman, J, Ilkayeva, O, Sarria, C, Zizzi, A, Wootton, J, Poppe, L, Anderson, P, Arlotto, M, Wei, Z, Granek, JA, Valdivia, RH, David, LA, Dressman, HK, Newgard, CB, Shah, SH, Seed, PC, Rawls, JF & Armstrong, SC 2021, 'The Pediatric Obesity Microbiome and Metabolism Study (POMMS): Methods, Baseline Data, and Early Insights', Obesity, vol. 29, no. 3, pp. 569-578. https://doi.org/10.1002/oby.23081

TY - JOUR

T1 - The Pediatric Obesity Microbiome and Metabolism Study (POMMS)

T2 - Methods, Baseline Data, and Early Insights

AU - McCann, Jessica R.

AU - Bihlmeyer, Nathan A.

AU - Roche, Kimberly

AU - Catherine, Cameron

AU - Jawahar, Jayanth

AU - Kwee, Lydia Coulter

AU - Younge, Noelle E.

AU - Silverman, Justin

AU - Ilkayeva, Olga

AU - Sarria, Charles

AU - Zizzi, Alexandra

AU - Wootton, Janet

AU - Poppe, Lisa

AU - Anderson, Paul

AU - Arlotto, Michelle

AU - Wei, Zhengzheng

AU - Granek, Joshua A.

AU - Valdivia, Raphael H.

AU - David, Lawrence A.

AU - Dressman, Holly K.

AU - Newgard, Christopher B.

AU - Shah, Svati H.

AU - Seed, Patrick C.

AU - Rawls, John F.

AU - Armstrong, Sarah C.

PY - 2021/3

Y1 - 2021/3

N2 - Objective: The purpose of this study was to establish a biorepository of clinical, metabolomic, and microbiome samples from adolescents with obesity as they undergo lifestyle modification. Methods: A total of 223 adolescents aged 10 to 18 years with BMI ≥95th percentile were enrolled, along with 71 healthy weight participants. Clinical data, fasting serum, and fecal samples were collected at repeated intervals over 6 months. Herein, the study design, data collection methods, and interim analysis—including targeted serum metabolite measurements and fecal 16S ribosomal RNA gene amplicon sequencing among adolescents with obesity (n = 27) and healthy weight controls (n = 27)—are presented. Results: Adolescents with obesity have higher serum alanine aminotransferase, C-reactive protein, and glycated hemoglobin, and they have lower high-density lipoprotein cholesterol when compared with healthy weight controls. Metabolomics revealed differences in branched-chain amino acid–related metabolites. Also observed was a differential abundance of specific microbial taxa and lower species diversity among adolescents with obesity when compared with the healthy weight group. Conclusions: The Pediatric Metabolism and Microbiome Study (POMMS) biorepository is available as a shared resource. Early findings suggest evidence of a metabolic signature of obesity unique to adolescents, along with confirmation of previously reported findings that describe metabolic and microbiome markers of obesity.

AB - Objective: The purpose of this study was to establish a biorepository of clinical, metabolomic, and microbiome samples from adolescents with obesity as they undergo lifestyle modification. Methods: A total of 223 adolescents aged 10 to 18 years with BMI ≥95th percentile were enrolled, along with 71 healthy weight participants. Clinical data, fasting serum, and fecal samples were collected at repeated intervals over 6 months. Herein, the study design, data collection methods, and interim analysis—including targeted serum metabolite measurements and fecal 16S ribosomal RNA gene amplicon sequencing among adolescents with obesity (n = 27) and healthy weight controls (n = 27)—are presented. Results: Adolescents with obesity have higher serum alanine aminotransferase, C-reactive protein, and glycated hemoglobin, and they have lower high-density lipoprotein cholesterol when compared with healthy weight controls. Metabolomics revealed differences in branched-chain amino acid–related metabolites. Also observed was a differential abundance of specific microbial taxa and lower species diversity among adolescents with obesity when compared with the healthy weight group. Conclusions: The Pediatric Metabolism and Microbiome Study (POMMS) biorepository is available as a shared resource. Early findings suggest evidence of a metabolic signature of obesity unique to adolescents, along with confirmation of previously reported findings that describe metabolic and microbiome markers of obesity.

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DO - 10.1002/oby.23081

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JO - Obesity

JF - Obesity

IS - 3

ER -

The Pediatric Obesity Microbiome and Metabolism Study (POMMS): Methods, Baseline Data, and Early Insights

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