TY - JOUR
T1 - The peroxisome proliferation-associated polypeptide in rat liver
AU - Reddy, J. K.
AU - Kumar, N. S.
N1 - Funding Information:
ACKNOWLEDGEMENT: This research was supported by the United States Public Health Service Grant GM-23750.
PY - 1977/8/8
Y1 - 1977/8/8
N2 - A marked increase in the content of a polypeptide, with an apparent molecular weight of 80,000, was observed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, in the post-nuclear, large particle (crude mitochondrial) and microsomal pellets of liver of rats treated with the hepatic peroxisome proliferators, clofibrate, nafenopin and Wy-14,643. This protein band was also present when peroxisome proliferation was induced in the absence of catalase synthesis, by combined treatment of rats with Wy-14,643 and the inhibitor of catalase synthesis, allylisopropylacetamide; suggesting that it is not a catalase subunit. No increase in this polypeptide occurred in rats treated with phenobarbital or with allylisopropylacetamide, the compounds which cause a marked proliferation of smooth endoplasmie reticulum in liver cells. The nature and significance of the increase of this polypeptide in the liver of rats treated with hypolipidemic peroxisome proliferators and its specificity, if any, to peroxisome membranes remain to be elucidated.
AB - A marked increase in the content of a polypeptide, with an apparent molecular weight of 80,000, was observed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, in the post-nuclear, large particle (crude mitochondrial) and microsomal pellets of liver of rats treated with the hepatic peroxisome proliferators, clofibrate, nafenopin and Wy-14,643. This protein band was also present when peroxisome proliferation was induced in the absence of catalase synthesis, by combined treatment of rats with Wy-14,643 and the inhibitor of catalase synthesis, allylisopropylacetamide; suggesting that it is not a catalase subunit. No increase in this polypeptide occurred in rats treated with phenobarbital or with allylisopropylacetamide, the compounds which cause a marked proliferation of smooth endoplasmie reticulum in liver cells. The nature and significance of the increase of this polypeptide in the liver of rats treated with hypolipidemic peroxisome proliferators and its specificity, if any, to peroxisome membranes remain to be elucidated.
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U2 - 10.1016/S0006-291X(77)80052-1
DO - 10.1016/S0006-291X(77)80052-1
M3 - Article
C2 - 901515
AN - SCOPUS:0017376192
VL - 77
SP - 824
EP - 829
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -