A marked increase in the content of a polypeptide, with an apparent molecular weight of 80,000, was observed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, in the post-nuclear, large particle (crude mitochondrial) and microsomal pellets of liver of rats treated with the hepatic peroxisome proliferators, clofibrate, nafenopin and Wy-14,643. This protein band was also present when peroxisome proliferation was induced in the absence of catalase synthesis, by combined treatment of rats with Wy-14,643 and the inhibitor of catalase synthesis, allylisopropylacetamide; suggesting that it is not a catalase subunit. No increase in this polypeptide occurred in rats treated with phenobarbital or with allylisopropylacetamide, the compounds which cause a marked proliferation of smooth endoplasmie reticulum in liver cells. The nature and significance of the increase of this polypeptide in the liver of rats treated with hypolipidemic peroxisome proliferators and its specificity, if any, to peroxisome membranes remain to be elucidated.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Aug 8 1977|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology