The phage 434 Cro OR1 complex at 2.5 Å resolution

A. Mondragón*, S. C. Harrison

*Corresponding author for this work

Research output: Contribution to journalArticle

128 Scopus citations

Abstract

The crystal structure of phage 434 Cro protein in complex with a 20 base-pair DNA fragment has been determined to 2.5 Å resolution. The DNA fragment contains the sequence of the OR1 operator site. The structure shows a bent conformation for the DNA, straighter at the center and more bent at the ends. The central base-pairs adopt conformations with significant deviations from coplanarity. The two molecules interact extensively along their common interface, both through hydrogen bonds and van der Waals interactions. The significance of these interactions for operator binding and recognition is discussed.

Original languageEnglish (US)
Pages (from-to)321-334
Number of pages14
JournalJournal of Molecular Biology
Volume219
Issue number2
DOIs
StatePublished - May 20 1991

Keywords

  • DNA conformation
  • DNA recognition
  • X-ray crystallography
  • helix-turn-helix
  • repressor

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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