The Pla protease of Yersinia pestis degrades Fas ligand to manipulate host cell death and inflammation

Adam J. Caulfield, Margaret E. Walker, Lindsay M. Gielda, Wyndham W. Lathem*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Pneumonic plague is a deadly respiratory disease caused by Yersinia pestis. The bacterial protease Pla contributes to disease progression and manipulation of host immunity, but the mechanisms by which this occurs are largely unknown. Here we show that Pla degrades the apoptotic signaling molecule Fas ligand (FasL) to prevent host cell apoptosis and inflammation. Wild-type Y. pestis, but not a Pla mutant (Δpla), degrades FasL, which results in decreased downstream caspase-3/7 activation and reduced apoptosis. Similarly, lungs of mice challenged with wild-type Y. pestis show reduced levels of FasL and activated caspase-3/7 compared to Δpla infection. Consistent with a role for FasL in regulating immune responses, Δpla infection results in aberrant proinflammatory cytokine levels. The loss of FasL or inhibition of caspase activity alters host inflammatory responses and enables enhanced Y. pestis outgrowth in the lungs. Thus, by degrading FasL, Y. pestis manipulates host cell death pathways to facilitate infection.

Original languageEnglish (US)
Pages (from-to)424-434
Number of pages11
JournalCell Host and Microbe
Volume15
Issue number4
DOIs
StatePublished - Apr 9 2014

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

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