The potential of laser scanning cytometry for early warning of algal blooms in desalination plant feedwater

Derek R. Vardon, Mark M. Clark, David A. Ladner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

When algal blooms (such as red tide) occur, early detection of increased algal growth in intake water offers desalination plant operators advanced warning of severe fouling and provides the necessary time for preventative measures. Monitoring basic water quality measurements such as turbidity, silt density index (SDI), dissolved oxygen, and bulk fluorescence can approximate algal activity; however, these measurements lack the resolution needed to accurately track growth throughout all phases of a bloom event. This study investigates the use of a laser scanning cytometer (LSC) that detects algal cells over a wide range of concentrations (50-150,000. cells/ml), and characterizes cell shape, size, and distribution parameters through fluorescence signal imaging. Water samples are filtered to collect algae on a 0.2-μm membrane surface. Sample volume is variable so cells can be concentrated. The membrane surface is scanned with a 635-nm diode laser and the signal is processed to generate a cell count and distribution image. LSC performance was comparable to fluorescence microscopy and flow cytometry over a range of concentrations that may be encountered during a bloom event. The applicability of LSC was demonstrated using laboratory-grown cultures and seawater samples taken during a bloom event off of the coast of Long Beach, CA.

Original languageEnglish (US)
Pages (from-to)193-200
Number of pages8
JournalDesalination
Volume277
Issue number1-3
DOIs
StatePublished - Aug 15 2011

Keywords

  • Algal bloom
  • Algal monitoring
  • Laser scanning cytometry
  • Seawater desalination
  • Solid phase cytometry

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)
  • Materials Science(all)
  • Water Science and Technology
  • Mechanical Engineering

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