The presence of a keratan sulfate-like glycoconjugate in microsomes of the cerebral cortex

Hyman G. Weinstein; Lawrence J. Blacik; Moira Breen; Paul A. Knepper

doi:10.1016/S0006-291X(83)80112-0

The presence of a keratan sulfate-like glycoconjugate in microsomes of the cerebral cortex

Hyman G. Weinstein, Lawrence J. Blacik, Moira Breen^*, Paul A. Knepper

^*Corresponding author for this work

Ophthalmology

Research output: Contribution to journal › Article › peer-review

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Abstract

Density gradient centrifugation of a 4 M guanidine hydrochloride preparation of the cerebral cortex showed that most of the keratan sulfate-like glycoconjugate was bound to the microsomes; the latter also contained heparan sulfate and one-third of the chondroitin sulfate proteoglycans. A hyaluronate-containing chondroitin sulfate proteoglycan was present in the soluble fraction.

Original language	English (US)
Pages (from-to)	28-35
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	111
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(83)80112-0
State	Published - Feb 28 1983

Funding

ACKNOWLEDGMENTS: This work was supported by the U.S. Veterans Administration and a grant from the National Institutes of Health, Institute on Aging, #2-ROI AG00902-04AI. The authors gratefully acknowledge the gifts of glycosaminoglycan reference standards from Dr. Martin B. Mathews, University of Chicago, prepared under grant from National Institutes of Health, and endo-B-galactosidase from Dr. S. Suzuki, Nagoya University. The authors thank Ms. Dianne Lewandowski and Dr. Bebe M. Baltrus for technical assistance, and Ms. Rita Heinz for editorial support.

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(83)80112-0

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title = "The presence of a keratan sulfate-like glycoconjugate in microsomes of the cerebral cortex",

abstract = "Density gradient centrifugation of a 4 M guanidine hydrochloride preparation of the cerebral cortex showed that most of the keratan sulfate-like glycoconjugate was bound to the microsomes; the latter also contained heparan sulfate and one-third of the chondroitin sulfate proteoglycans. A hyaluronate-containing chondroitin sulfate proteoglycan was present in the soluble fraction.",

author = "Weinstein, {Hyman G.} and Blacik, {Lawrence J.} and Moira Breen and Knepper, {Paul A.}",

note = "Funding Information: ACKNOWLEDGMENTS: This work was supported by the U.S. Veterans Administration and a grant from the National Institutes of Health, Institute on Aging, #2-ROI AG00902-04AI. The authors gratefully acknowledge the gifts of glycosaminoglycan reference standards from Dr. Martin B. Mathews, University of Chicago, prepared under grant from National Institutes of Health, and endo-B-galactosidase from Dr. S. Suzuki, Nagoya University. The authors thank Ms. Dianne Lewandowski and Dr. Bebe M. Baltrus for technical assistance, and Ms. Rita Heinz for editorial support.",

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AU - Breen, Moira

AU - Knepper, Paul A.

N1 - Funding Information: ACKNOWLEDGMENTS: This work was supported by the U.S. Veterans Administration and a grant from the National Institutes of Health, Institute on Aging, #2-ROI AG00902-04AI. The authors gratefully acknowledge the gifts of glycosaminoglycan reference standards from Dr. Martin B. Mathews, University of Chicago, prepared under grant from National Institutes of Health, and endo-B-galactosidase from Dr. S. Suzuki, Nagoya University. The authors thank Ms. Dianne Lewandowski and Dr. Bebe M. Baltrus for technical assistance, and Ms. Rita Heinz for editorial support.

PY - 1983/2/28

Y1 - 1983/2/28

N2 - Density gradient centrifugation of a 4 M guanidine hydrochloride preparation of the cerebral cortex showed that most of the keratan sulfate-like glycoconjugate was bound to the microsomes; the latter also contained heparan sulfate and one-third of the chondroitin sulfate proteoglycans. A hyaluronate-containing chondroitin sulfate proteoglycan was present in the soluble fraction.

AB - Density gradient centrifugation of a 4 M guanidine hydrochloride preparation of the cerebral cortex showed that most of the keratan sulfate-like glycoconjugate was bound to the microsomes; the latter also contained heparan sulfate and one-third of the chondroitin sulfate proteoglycans. A hyaluronate-containing chondroitin sulfate proteoglycan was present in the soluble fraction.

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The presence of a keratan sulfate-like glycoconjugate in microsomes of the cerebral cortex

Abstract

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ASJC Scopus subject areas

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