The presence of a keratan sulfate-like glycoconjugate in microsomes of the cerebral cortex

Hyman G. Weinstein, Lawrence J. Blacik, Moira Breen*, Paul A. Knepper

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Density gradient centrifugation of a 4 M guanidine hydrochloride preparation of the cerebral cortex showed that most of the keratan sulfate-like glycoconjugate was bound to the microsomes; the latter also contained heparan sulfate and one-third of the chondroitin sulfate proteoglycans. A hyaluronate-containing chondroitin sulfate proteoglycan was present in the soluble fraction.

Original languageEnglish (US)
Pages (from-to)28-35
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume111
Issue number1
DOIs
StatePublished - Feb 28 1983

Funding

ACKNOWLEDGMENTS: This work was supported by the U.S. Veterans Administration and a grant from the National Institutes of Health, Institute on Aging, #2-ROI AG00902-04AI. The authors gratefully acknowledge the gifts of glycosaminoglycan reference standards from Dr. Martin B. Mathews, University of Chicago, prepared under grant from National Institutes of Health, and endo-B-galactosidase from Dr. S. Suzuki, Nagoya University. The authors thank Ms. Dianne Lewandowski and Dr. Bebe M. Baltrus for technical assistance, and Ms. Rita Heinz for editorial support.

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'The presence of a keratan sulfate-like glycoconjugate in microsomes of the cerebral cortex'. Together they form a unique fingerprint.

Cite this