Abstract
Density gradient centrifugation of a 4 M guanidine hydrochloride preparation of the cerebral cortex showed that most of the keratan sulfate-like glycoconjugate was bound to the microsomes; the latter also contained heparan sulfate and one-third of the chondroitin sulfate proteoglycans. A hyaluronate-containing chondroitin sulfate proteoglycan was present in the soluble fraction.
Original language | English (US) |
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Pages (from-to) | 28-35 |
Number of pages | 8 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 111 |
Issue number | 1 |
DOIs | |
State | Published - Feb 28 1983 |
Funding
ACKNOWLEDGMENTS: This work was supported by the U.S. Veterans Administration and a grant from the National Institutes of Health, Institute on Aging, #2-ROI AG00902-04AI. The authors gratefully acknowledge the gifts of glycosaminoglycan reference standards from Dr. Martin B. Mathews, University of Chicago, prepared under grant from National Institutes of Health, and endo-B-galactosidase from Dr. S. Suzuki, Nagoya University. The authors thank Ms. Dianne Lewandowski and Dr. Bebe M. Baltrus for technical assistance, and Ms. Rita Heinz for editorial support.
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology