The presence of transcription factors in chicken albumin, yolk and blastoderm

Paul A. Knepper*, Chandra S K Mayanil, Erin Hayes, William Goossens, Richard W. Byrne, David G. Mclone

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Embryonic development is determined by preset intrinsic programs and extrinsic signals. To explore the possibility that transcription factors are present at the onset of development, preparations of yolk, albumin, and blastoderm from unfertilized and fertilized white Leghorn chicken eggs were screened by a panel of 16 transcription factor antibodies with Western blot techniques. Yolk was positive for 13 transcription factors, whereas blastoderm was positive for 10, and albumin was positive for 5. In yolk, several transcription factors, GATA-2, E2F-1, MyoD, and TFIID, were developmentally regulated. These results indicate that intracellular yolk and extracellular albumin contain transcription factors which presumably influence early chick embryonic development from prefertilization to the late blastoderm stage. Thus, the utility of preset maternal transcription factors within yolk and albumin complement maternally derived mRNA to determine the early development of the zygote.

Original languageEnglish (US)
Pages (from-to)357-363
Number of pages7
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume35
Issue number6
DOIs
StatePublished - Jan 1 1999

Keywords

  • Albumin
  • DNA binding proteins
  • Transcriptions factors
  • Western blots
  • Yolk

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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