The presence of transcription factors in fetal bovine sera

Paul A. Knepper*

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Three sources of fetal bovine serum (FBS) were fractionated by ammonium sulfate precipitation and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to Immobilon-P membranes, immunoblotted with a panel of transcription factor antibodies, and detected by enhanced chemiluminescence. Nine transcription factors were detected-ATF- 2, SRE-ZBP, GATA-2, TFIID, Ets-1/Ets-2, E2F-1, Oct-2, p53, and AP-2; four transcription factors were not detected-Myo D, CREB, Sp2, and Wilms' tumor. The results indicated the presence of varying amounts of several transcription factors in three commercial sources and may represent heretofore unrecognized factors influencing cell culture.

Original languageEnglish (US)
Pages (from-to)170-173
Number of pages4
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume34
Issue number2
DOIs
StatePublished - Jan 1 1998

Keywords

  • Ammonium sulfate precipitation
  • DNA binding proteins
  • Fetal bovine serum
  • SDS-PAGE
  • Transcription factors
  • Western blots

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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