The presence of transcription factors in fetal bovine sera

Paul A. Knepper

doi:10.1007/s11626-998-0101-2

The presence of transcription factors in fetal bovine sera

Paul A. Knepper^*

^*Corresponding author for this work

Ophthalmology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Three sources of fetal bovine serum (FBS) were fractionated by ammonium sulfate precipitation and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to Immobilon-P membranes, immunoblotted with a panel of transcription factor antibodies, and detected by enhanced chemiluminescence. Nine transcription factors were detected-ATF- 2, SRE-ZBP, GATA-2, TFIID, Ets-1/Ets-2, E2F-1, Oct-2, p53, and AP-2; four transcription factors were not detected-Myo D, CREB, Sp2, and Wilms' tumor. The results indicated the presence of varying amounts of several transcription factors in three commercial sources and may represent heretofore unrecognized factors influencing cell culture.

Original language	English (US)
Pages (from-to)	170-173
Number of pages	4
Journal	In Vitro Cellular and Developmental Biology - Animal
Volume	34
Issue number	2
DOIs	https://doi.org/10.1007/s11626-998-0101-2
State	Published - 1998

Keywords

Ammonium sulfate precipitation
DNA binding proteins
Fetal bovine serum
SDS-PAGE
Transcription factors
Western blots

ASJC Scopus subject areas

Cell Biology
Developmental Biology

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10.1007/s11626-998-0101-2

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title = "The presence of transcription factors in fetal bovine sera",

abstract = "Three sources of fetal bovine serum (FBS) were fractionated by ammonium sulfate precipitation and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to Immobilon-P membranes, immunoblotted with a panel of transcription factor antibodies, and detected by enhanced chemiluminescence. Nine transcription factors were detected-ATF- 2, SRE-ZBP, GATA-2, TFIID, Ets-1/Ets-2, E2F-1, Oct-2, p53, and AP-2; four transcription factors were not detected-Myo D, CREB, Sp2, and Wilms' tumor. The results indicated the presence of varying amounts of several transcription factors in three commercial sources and may represent heretofore unrecognized factors influencing cell culture.",

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AU - Knepper, Paul A.

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N2 - Three sources of fetal bovine serum (FBS) were fractionated by ammonium sulfate precipitation and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to Immobilon-P membranes, immunoblotted with a panel of transcription factor antibodies, and detected by enhanced chemiluminescence. Nine transcription factors were detected-ATF- 2, SRE-ZBP, GATA-2, TFIID, Ets-1/Ets-2, E2F-1, Oct-2, p53, and AP-2; four transcription factors were not detected-Myo D, CREB, Sp2, and Wilms' tumor. The results indicated the presence of varying amounts of several transcription factors in three commercial sources and may represent heretofore unrecognized factors influencing cell culture.

AB - Three sources of fetal bovine serum (FBS) were fractionated by ammonium sulfate precipitation and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to Immobilon-P membranes, immunoblotted with a panel of transcription factor antibodies, and detected by enhanced chemiluminescence. Nine transcription factors were detected-ATF- 2, SRE-ZBP, GATA-2, TFIID, Ets-1/Ets-2, E2F-1, Oct-2, p53, and AP-2; four transcription factors were not detected-Myo D, CREB, Sp2, and Wilms' tumor. The results indicated the presence of varying amounts of several transcription factors in three commercial sources and may represent heretofore unrecognized factors influencing cell culture.

KW - Ammonium sulfate precipitation

KW - DNA binding proteins

KW - Fetal bovine serum

KW - SDS-PAGE

KW - Transcription factors

KW - Western blots

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The presence of transcription factors in fetal bovine sera

Abstract

Keywords

ASJC Scopus subject areas

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