TY - JOUR
T1 - The pseudorabies virus R2 non-neuroinvasive vaccine
T2 - A proof-of-concept study in pigs
AU - Pickard, Gary E.
AU - Brodersen, Bruce
AU - Sollars, Patricia J.
AU - Smith, Gregory A.
N1 - Funding Information:
We would like to acknowledge the excellent technical assistance of the staff of the Life Science Annex and the Veterinary Diagnostic Center, University of Nebraska-Lincoln. We also thank Kelsey Karnik and the UNL Statistical Cross-disciplinary Collaboration and Consulting Lab for assistance in the statistical analyses.
Publisher Copyright:
© 2020 The Authors
PY - 2020/6/15
Y1 - 2020/6/15
N2 - Neurotropic alpha-herpesviruses that infect mammals establish life-long latent infections in the peripheral nervous system after initial infection of exposed mucosal tissues. The neuroinvasive properties can lead to severe complications both with clinical and veterinary alpha-herpesviruses, and vaccines are often unavailable or provide limited protection. Here we assess the properties and efficacy of an R2 vaccine derived from the alpha-herpesvirus, pseudorabies virus (PRV), in pigs. We demonstrate that the PRV R2 vaccine does not invade the porcine peripheral nervous system within the limits of detection. Furthermore, after a single intranasal vaccination, R2 conferred protection to pigs subsequently challenged with a virulent PRV field strain (NIA-3). These findings support that the R2 vaccine design is non-neuroinvasive and is an effective vaccine in the context of a natural host.
AB - Neurotropic alpha-herpesviruses that infect mammals establish life-long latent infections in the peripheral nervous system after initial infection of exposed mucosal tissues. The neuroinvasive properties can lead to severe complications both with clinical and veterinary alpha-herpesviruses, and vaccines are often unavailable or provide limited protection. Here we assess the properties and efficacy of an R2 vaccine derived from the alpha-herpesvirus, pseudorabies virus (PRV), in pigs. We demonstrate that the PRV R2 vaccine does not invade the porcine peripheral nervous system within the limits of detection. Furthermore, after a single intranasal vaccination, R2 conferred protection to pigs subsequently challenged with a virulent PRV field strain (NIA-3). These findings support that the R2 vaccine design is non-neuroinvasive and is an effective vaccine in the context of a natural host.
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U2 - 10.1016/j.vaccine.2020.05.002
DO - 10.1016/j.vaccine.2020.05.002
M3 - Article
C2 - 32448623
AN - SCOPUS:85084976734
SN - 0264-410X
VL - 38
SP - 4524
EP - 4528
JO - Vaccine
JF - Vaccine
IS - 29
ER -
