The putative protein 6 of the severe acute respiratory syndrome-associated coronavirus: Expression and functional characterization

Hua Geng; Yuet Man Liu; Wai Sing Chan; Anthony Wing Ip Lo; Deborah Mun Yee Au; Mary Miu Yee Waye; Yuan Yuan Ho

doi:10.1016/j.febslet.2005.11.007

The putative protein 6 of the severe acute respiratory syndrome-associated coronavirus: Expression and functional characterization

Hua Geng, Yuet Man Liu, Wai Sing Chan, Anthony Wing Ip Lo, Deborah Mun Yee Au, Mary Miu Yee Waye, Yuan Yuan Ho^*

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The SARS-CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS-CoV infected Vero E6 cells by immunohistochemistry. Further studies by immunoblot and confocal microscopy analyses revealed the expression and the endoplasmic reticulum (ER) localization of the recombinant SARS 6 protein in mammalian cells. Expression of SARS 6 protein in mammalian cells elicits biological activity of stimulating cellular DNA synthesis.

Original language	English (US)
Pages (from-to)	6763-6768
Number of pages	6
Journal	FEBS Letters
Volume	579
Issue number	30
DOIs	https://doi.org/10.1016/j.febslet.2005.11.007
State	Published - Dec 19 2005

Keywords

Coronavirus
ORF6
SARS 6 protein
SARS-CoV
Severe acute respiratory syndrome

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "The putative protein 6 of the severe acute respiratory syndrome-associated coronavirus: Expression and functional characterization",

abstract = "The SARS-CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS-CoV infected Vero E6 cells by immunohistochemistry. Further studies by immunoblot and confocal microscopy analyses revealed the expression and the endoplasmic reticulum (ER) localization of the recombinant SARS 6 protein in mammalian cells. Expression of SARS 6 protein in mammalian cells elicits biological activity of stimulating cellular DNA synthesis.",

keywords = "Coronavirus, ORF6, SARS 6 protein, SARS-CoV, Severe acute respiratory syndrome",

author = "Hua Geng and Liu, {Yuet Man} and Chan, {Wai Sing} and Lo, {Anthony Wing Ip} and Au, {Deborah Mun Yee} and Waye, {Mary Miu Yee} and Ho, {Yuan Yuan}",

note = "Funding Information: This work was supported in part by the Research Fund for the Control of Infectious Disease, Health, Welfare and Food Bureau, Hong Kong (Ref. No. 01030592); the RGC Special SARS grant (Ref. No. CUHK 4536/03M); Direct Grant, Faculty of Medicine, The Chinese University of Hong Kong, and the Croucher Foundation. We thank Dr. K.C. Choi at the Center for Epidemiology and Biostatistics, The Chinese University of Hong Kong, for the statistical analyses. And we thank Professor C.K. Wong and his student P.W. Li at the Department of Chemical Pathology, The Chinese University of Hong Kong, for their technical support.",

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doi = "10.1016/j.febslet.2005.11.007",

language = "English (US)",

volume = "579",

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The putative protein 6 of the severe acute respiratory syndrome-associated coronavirus : Expression and functional characterization. / Geng, Hua; Liu, Yuet Man; Chan, Wai Sing; Lo, Anthony Wing Ip; Au, Deborah Mun Yee; Waye, Mary Miu Yee; Ho, Yuan Yuan.

In: FEBS Letters, Vol. 579, No. 30, 19.12.2005, p. 6763-6768.

Research output: Contribution to journal › Article › peer-review

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T1 - The putative protein 6 of the severe acute respiratory syndrome-associated coronavirus

T2 - Expression and functional characterization

AU - Geng, Hua

AU - Liu, Yuet Man

AU - Chan, Wai Sing

AU - Lo, Anthony Wing Ip

AU - Au, Deborah Mun Yee

AU - Waye, Mary Miu Yee

AU - Ho, Yuan Yuan

N1 - Funding Information: This work was supported in part by the Research Fund for the Control of Infectious Disease, Health, Welfare and Food Bureau, Hong Kong (Ref. No. 01030592); the RGC Special SARS grant (Ref. No. CUHK 4536/03M); Direct Grant, Faculty of Medicine, The Chinese University of Hong Kong, and the Croucher Foundation. We thank Dr. K.C. Choi at the Center for Epidemiology and Biostatistics, The Chinese University of Hong Kong, for the statistical analyses. And we thank Professor C.K. Wong and his student P.W. Li at the Department of Chemical Pathology, The Chinese University of Hong Kong, for their technical support.

PY - 2005/12/19

Y1 - 2005/12/19

N2 - The SARS-CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS-CoV infected Vero E6 cells by immunohistochemistry. Further studies by immunoblot and confocal microscopy analyses revealed the expression and the endoplasmic reticulum (ER) localization of the recombinant SARS 6 protein in mammalian cells. Expression of SARS 6 protein in mammalian cells elicits biological activity of stimulating cellular DNA synthesis.

AB - The SARS-CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS-CoV infected Vero E6 cells by immunohistochemistry. Further studies by immunoblot and confocal microscopy analyses revealed the expression and the endoplasmic reticulum (ER) localization of the recombinant SARS 6 protein in mammalian cells. Expression of SARS 6 protein in mammalian cells elicits biological activity of stimulating cellular DNA synthesis.

KW - Coronavirus

KW - ORF6

KW - SARS 6 protein

KW - SARS-CoV

KW - Severe acute respiratory syndrome

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The putative protein 6 of the severe acute respiratory syndrome-associated coronavirus: Expression and functional characterization

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ASJC Scopus subject areas

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