Abstract
The SARS-CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS-CoV infected Vero E6 cells by immunohistochemistry. Further studies by immunoblot and confocal microscopy analyses revealed the expression and the endoplasmic reticulum (ER) localization of the recombinant SARS 6 protein in mammalian cells. Expression of SARS 6 protein in mammalian cells elicits biological activity of stimulating cellular DNA synthesis.
Original language | English (US) |
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Pages (from-to) | 6763-6768 |
Number of pages | 6 |
Journal | FEBS Letters |
Volume | 579 |
Issue number | 30 |
DOIs | |
State | Published - Dec 19 2005 |
Keywords
- Coronavirus
- ORF6
- SARS 6 protein
- SARS-CoV
- Severe acute respiratory syndrome
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology