The putative protein 6 of the severe acute respiratory syndrome-associated coronavirus: Expression and functional characterization

Hua Geng, Yuet Man Liu, Wai Sing Chan, Anthony Wing Ip Lo, Deborah Mun Yee Au, Mary Miu Yee Waye, Yuan Yuan Ho*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The SARS-CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS-CoV infected Vero E6 cells by immunohistochemistry. Further studies by immunoblot and confocal microscopy analyses revealed the expression and the endoplasmic reticulum (ER) localization of the recombinant SARS 6 protein in mammalian cells. Expression of SARS 6 protein in mammalian cells elicits biological activity of stimulating cellular DNA synthesis.

Original languageEnglish (US)
Pages (from-to)6763-6768
Number of pages6
JournalFEBS Letters
Volume579
Issue number30
DOIs
StatePublished - Dec 19 2005

Keywords

  • Coronavirus
  • ORF6
  • SARS 6 protein
  • SARS-CoV
  • Severe acute respiratory syndrome

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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