The RecF protein antagonizes RecX function via direct interaction

Shelley L. Lusetti, Michael D. Hobbs, Elizabeth A. Stohl, Sindhu Chitteni-Pattu, Ross B. Inman, H. Steven Seifert, Michael M. Cox*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

60 Scopus citations


The RecX protein inhibits RecA filament extension, leading to net filament disassembly. The RecF protein physically interacts with the RecX protein and protects RecA from the inhibitory effects of RecX. In vitro, efficient RecA filament formation onto single-stranded DNA binding protein (SSB)-coated circular single-stranded DNA (ssDNA) in the presence of RecX occurs only when all of the RecFOR proteins are present. The RecOR proteins contribute only to RecA filament nucleation onto SSB-coated single-stranded DNA and are unable to counter the inhibitory effects of RecX on RecA filaments. RecF protein uniquely supports substantial RecA filament extension in the presence of RecX. In vivo, RecF protein counters a RecX-mediated inhibition of plasmid recombination. Thus, a significant positive contribution of RecF to RecA filament assembly is to antagonize the effects of the negative modulator RecX, specifically during the extension phase.

Original languageEnglish (US)
Pages (from-to)41-50
Number of pages10
JournalMolecular cell
Issue number1
StatePublished - Jan 6 2006

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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