The RecF protein antagonizes RecX function via direct interaction

Shelley L. Lusetti; Michael D. Hobbs; Elizabeth A. Stohl; Sindhu Chitteni-Pattu; Ross B. Inman; H. Steven Seifert; Michael M. Cox

doi:10.1016/j.molcel.2005.11.011

The RecF protein antagonizes RecX function via direct interaction

Shelley L. Lusetti, Michael D. Hobbs, Elizabeth A. Stohl, Sindhu Chitteni-Pattu, Ross B. Inman, H. Steven Seifert, Michael M. Cox^*

^*Corresponding author for this work

Microbiology-Immunology

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

The RecX protein inhibits RecA filament extension, leading to net filament disassembly. The RecF protein physically interacts with the RecX protein and protects RecA from the inhibitory effects of RecX. In vitro, efficient RecA filament formation onto single-stranded DNA binding protein (SSB)-coated circular single-stranded DNA (ssDNA) in the presence of RecX occurs only when all of the RecFOR proteins are present. The RecOR proteins contribute only to RecA filament nucleation onto SSB-coated single-stranded DNA and are unable to counter the inhibitory effects of RecX on RecA filaments. RecF protein uniquely supports substantial RecA filament extension in the presence of RecX. In vivo, RecF protein counters a RecX-mediated inhibition of plasmid recombination. Thus, a significant positive contribution of RecF to RecA filament assembly is to antagonize the effects of the negative modulator RecX, specifically during the extension phase.

Original language	English (US)
Pages (from-to)	41-50
Number of pages	10
Journal	Molecular cell
Volume	21
Issue number	1
DOIs	https://doi.org/10.1016/j.molcel.2005.11.011
State	Published - Jan 6 2006

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/j.molcel.2005.11.011

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@article{e11fa12229f24ae3b8abc7829c6fbc56,

title = "The RecF protein antagonizes RecX function via direct interaction",

abstract = "The RecX protein inhibits RecA filament extension, leading to net filament disassembly. The RecF protein physically interacts with the RecX protein and protects RecA from the inhibitory effects of RecX. In vitro, efficient RecA filament formation onto single-stranded DNA binding protein (SSB)-coated circular single-stranded DNA (ssDNA) in the presence of RecX occurs only when all of the RecFOR proteins are present. The RecOR proteins contribute only to RecA filament nucleation onto SSB-coated single-stranded DNA and are unable to counter the inhibitory effects of RecX on RecA filaments. RecF protein uniquely supports substantial RecA filament extension in the presence of RecX. In vivo, RecF protein counters a RecX-mediated inhibition of plasmid recombination. Thus, a significant positive contribution of RecF to RecA filament assembly is to antagonize the effects of the negative modulator RecX, specifically during the extension phase.",

author = "Lusetti, {Shelley L.} and Hobbs, {Michael D.} and Stohl, {Elizabeth A.} and Sindhu Chitteni-Pattu and Inman, {Ross B.} and Seifert, {H. Steven} and Cox, {Michael M.}",

note = "Funding Information: This work was supported by National Institutes of Health grants GM52725 (to M.M.C.) and AI044239 (to H.S.S.), and by the National Institutes of Health Predoctoral Training Grant T32 GM007215 in Molecular Biosciences from the National Institute of General Medical Sciences (to M.D.H.).",

year = "2006",

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doi = "10.1016/j.molcel.2005.11.011",

language = "English (US)",

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T1 - The RecF protein antagonizes RecX function via direct interaction

AU - Lusetti, Shelley L.

AU - Hobbs, Michael D.

AU - Stohl, Elizabeth A.

AU - Chitteni-Pattu, Sindhu

AU - Inman, Ross B.

AU - Seifert, H. Steven

AU - Cox, Michael M.

N1 - Funding Information: This work was supported by National Institutes of Health grants GM52725 (to M.M.C.) and AI044239 (to H.S.S.), and by the National Institutes of Health Predoctoral Training Grant T32 GM007215 in Molecular Biosciences from the National Institute of General Medical Sciences (to M.D.H.).

PY - 2006/1/6

Y1 - 2006/1/6

N2 - The RecX protein inhibits RecA filament extension, leading to net filament disassembly. The RecF protein physically interacts with the RecX protein and protects RecA from the inhibitory effects of RecX. In vitro, efficient RecA filament formation onto single-stranded DNA binding protein (SSB)-coated circular single-stranded DNA (ssDNA) in the presence of RecX occurs only when all of the RecFOR proteins are present. The RecOR proteins contribute only to RecA filament nucleation onto SSB-coated single-stranded DNA and are unable to counter the inhibitory effects of RecX on RecA filaments. RecF protein uniquely supports substantial RecA filament extension in the presence of RecX. In vivo, RecF protein counters a RecX-mediated inhibition of plasmid recombination. Thus, a significant positive contribution of RecF to RecA filament assembly is to antagonize the effects of the negative modulator RecX, specifically during the extension phase.

AB - The RecX protein inhibits RecA filament extension, leading to net filament disassembly. The RecF protein physically interacts with the RecX protein and protects RecA from the inhibitory effects of RecX. In vitro, efficient RecA filament formation onto single-stranded DNA binding protein (SSB)-coated circular single-stranded DNA (ssDNA) in the presence of RecX occurs only when all of the RecFOR proteins are present. The RecOR proteins contribute only to RecA filament nucleation onto SSB-coated single-stranded DNA and are unable to counter the inhibitory effects of RecX on RecA filaments. RecF protein uniquely supports substantial RecA filament extension in the presence of RecX. In vivo, RecF protein counters a RecX-mediated inhibition of plasmid recombination. Thus, a significant positive contribution of RecF to RecA filament assembly is to antagonize the effects of the negative modulator RecX, specifically during the extension phase.

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JO - Molecular cell

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The RecF protein antagonizes RecX function via direct interaction

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