The regulation of tumor necrosis factor-α production in murine mast cells: Pentoxifylline or dexamethasone inhibits IgE-dependent production of TNF-α by distinct mechanisms

Anjona Schmidt-Choudhury, Glenn T. Furuta, Jackie A. Lavigne, Stephen J. Galli, Barry K. Wershil*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Mast cells activated via high-affinity receptors for IgE can produce a variety of multifunctional cytokines, including TNF-α, which is thought to be involved in the pathophysiology of allergic diseases and other inflammatory disorders. We investigated the regulation of Fc(ε)RI-dependent TNF-α production by mouse mast cells using dexamethasone and pentoxifylline, pharmacological agents which are known to suppress TNF-α production by macrophages. We now report that either dexamethasone or pentoxifylline can inhibit IgE-dependent mouse mast cell production of TNF- α; however, the major site of action of these agents was different. Pentoxifylline inhibited mast cell TNF-α gene transcription, while dexamethasone inhibited TNF-α production predominantly by a posttranscriptional mechanism. These results demonstrate that the synthesis of mast cell TNF-α can be regulated pharmacologically at either the transcriptional or the translational level and that pentoxifylline and dexamethasone, two agents that are used to treat inflammatory disorders, can modulate mast cell TNF-α production at different points in the synthetic pathway of this cytokine.

Original languageEnglish (US)
Pages (from-to)140-146
Number of pages7
JournalCellular Immunology
Volume171
Issue number1
DOIs
StatePublished - Jul 10 1996

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'The regulation of tumor necrosis factor-α production in murine mast cells: Pentoxifylline or dexamethasone inhibits IgE-dependent production of TNF-α by distinct mechanisms'. Together they form a unique fingerprint.

Cite this