The regulatory effect of electro-acupuncture on the expression of NMDA receptors in a SCI rat model

Wen Zhan Tu, Wen Ci Chen, Wan Xia, Rong He, Jie Hu, Mingchen Jiang, Song He Jiang*

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background In early spinal cord injury (SCI), glutamate receptors, including N-methyl-D-aspartate (NMDA) receptors (NMDARs), are over-stimulated by excessively released glutamate. The enhanced activity of NMDARs may cause cell death by overloading calcium (Ca2 +) into cells based on their high permeability to Ca2 +. Studies in SCI animals have shown that treatment with electro-acupuncture (EA) is able to reduce cell death and to improve functional recovery. One possible mechanism of this neuroprotective effect is that EA has regulatory effect on NMDARs. Aims To test whether EA could protect the spinal cord after SCI by decreasing the expression levels of NR1 and NR2A. Main methods We conducted EA treatment on a rat SCI model produced with a New York University (NYU) Impactor and measured hindlimb locomotor function by Basso, Beattie and Bresnahan Locomotor Rating Scale (BBB Scale). The expression of NR1 and NR2, the subunits of NMDARs, in the injured spinal cord was measured by Immunofluorescence stainings, western blot and real-time quantitative PCR (RT-qPCR). Key finding Our results showed that two days after the SCI the expression of NR1 and NR2 were dramatically enhanced at both protein and mNRA levels, which were significantly reduced by EA treatment at two specific acupoints, Dazhui (DU14) and Mingmen (DU4). Significance EA is a potential therapeutic method for treating early SCI in human.

Original languageEnglish (US)
Pages (from-to)8-14
Number of pages7
JournalLife Sciences
Volume177
DOIs
StatePublished - May 15 2017

Fingerprint

Electroacupuncture
Acupuncture
N-Methyl-D-Aspartate Receptors
Spinal Cord Injuries
Rats
Acupuncture Therapy
Spinal Cord
Cell Death
Cell death
Acupuncture Points
Glutamate Receptors
Neuroprotective Agents
Hindlimb
Fluorescent Antibody Technique
Real-Time Polymerase Chain Reaction
Glutamic Acid
Cause of Death
Permeability
Western Blotting
Staining and Labeling

Keywords

  • Electro-acupuncture
  • NMDA receptors
  • SCI
  • Therapy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Tu, Wen Zhan ; Chen, Wen Ci ; Xia, Wan ; He, Rong ; Hu, Jie ; Jiang, Mingchen ; Jiang, Song He. / The regulatory effect of electro-acupuncture on the expression of NMDA receptors in a SCI rat model. In: Life Sciences. 2017 ; Vol. 177. pp. 8-14.
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abstract = "Background In early spinal cord injury (SCI), glutamate receptors, including N-methyl-D-aspartate (NMDA) receptors (NMDARs), are over-stimulated by excessively released glutamate. The enhanced activity of NMDARs may cause cell death by overloading calcium (Ca2 +) into cells based on their high permeability to Ca2 +. Studies in SCI animals have shown that treatment with electro-acupuncture (EA) is able to reduce cell death and to improve functional recovery. One possible mechanism of this neuroprotective effect is that EA has regulatory effect on NMDARs. Aims To test whether EA could protect the spinal cord after SCI by decreasing the expression levels of NR1 and NR2A. Main methods We conducted EA treatment on a rat SCI model produced with a New York University (NYU) Impactor and measured hindlimb locomotor function by Basso, Beattie and Bresnahan Locomotor Rating Scale (BBB Scale). The expression of NR1 and NR2, the subunits of NMDARs, in the injured spinal cord was measured by Immunofluorescence stainings, western blot and real-time quantitative PCR (RT-qPCR). Key finding Our results showed that two days after the SCI the expression of NR1 and NR2 were dramatically enhanced at both protein and mNRA levels, which were significantly reduced by EA treatment at two specific acupoints, Dazhui (DU14) and Mingmen (DU4). Significance EA is a potential therapeutic method for treating early SCI in human.",
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The regulatory effect of electro-acupuncture on the expression of NMDA receptors in a SCI rat model. / Tu, Wen Zhan; Chen, Wen Ci; Xia, Wan; He, Rong; Hu, Jie; Jiang, Mingchen; Jiang, Song He.

In: Life Sciences, Vol. 177, 15.05.2017, p. 8-14.

Research output: Contribution to journalArticle

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T1 - The regulatory effect of electro-acupuncture on the expression of NMDA receptors in a SCI rat model

AU - Tu, Wen Zhan

AU - Chen, Wen Ci

AU - Xia, Wan

AU - He, Rong

AU - Hu, Jie

AU - Jiang, Mingchen

AU - Jiang, Song He

PY - 2017/5/15

Y1 - 2017/5/15

N2 - Background In early spinal cord injury (SCI), glutamate receptors, including N-methyl-D-aspartate (NMDA) receptors (NMDARs), are over-stimulated by excessively released glutamate. The enhanced activity of NMDARs may cause cell death by overloading calcium (Ca2 +) into cells based on their high permeability to Ca2 +. Studies in SCI animals have shown that treatment with electro-acupuncture (EA) is able to reduce cell death and to improve functional recovery. One possible mechanism of this neuroprotective effect is that EA has regulatory effect on NMDARs. Aims To test whether EA could protect the spinal cord after SCI by decreasing the expression levels of NR1 and NR2A. Main methods We conducted EA treatment on a rat SCI model produced with a New York University (NYU) Impactor and measured hindlimb locomotor function by Basso, Beattie and Bresnahan Locomotor Rating Scale (BBB Scale). The expression of NR1 and NR2, the subunits of NMDARs, in the injured spinal cord was measured by Immunofluorescence stainings, western blot and real-time quantitative PCR (RT-qPCR). Key finding Our results showed that two days after the SCI the expression of NR1 and NR2 were dramatically enhanced at both protein and mNRA levels, which were significantly reduced by EA treatment at two specific acupoints, Dazhui (DU14) and Mingmen (DU4). Significance EA is a potential therapeutic method for treating early SCI in human.

AB - Background In early spinal cord injury (SCI), glutamate receptors, including N-methyl-D-aspartate (NMDA) receptors (NMDARs), are over-stimulated by excessively released glutamate. The enhanced activity of NMDARs may cause cell death by overloading calcium (Ca2 +) into cells based on their high permeability to Ca2 +. Studies in SCI animals have shown that treatment with electro-acupuncture (EA) is able to reduce cell death and to improve functional recovery. One possible mechanism of this neuroprotective effect is that EA has regulatory effect on NMDARs. Aims To test whether EA could protect the spinal cord after SCI by decreasing the expression levels of NR1 and NR2A. Main methods We conducted EA treatment on a rat SCI model produced with a New York University (NYU) Impactor and measured hindlimb locomotor function by Basso, Beattie and Bresnahan Locomotor Rating Scale (BBB Scale). The expression of NR1 and NR2, the subunits of NMDARs, in the injured spinal cord was measured by Immunofluorescence stainings, western blot and real-time quantitative PCR (RT-qPCR). Key finding Our results showed that two days after the SCI the expression of NR1 and NR2 were dramatically enhanced at both protein and mNRA levels, which were significantly reduced by EA treatment at two specific acupoints, Dazhui (DU14) and Mingmen (DU4). Significance EA is a potential therapeutic method for treating early SCI in human.

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