TY - JOUR
T1 - The relationship between reticulated platelets, intestinal alkaline phosphatase, and necrotizing enterocolitis
AU - Kampanatkosol, Richard
AU - Thomson, Tricia
AU - Habeeb, Omar
AU - Glynn, Loretto
AU - Dechristopher, Phillip J.
AU - Yong, Sherri
AU - Jeske, Walter
AU - Maheshwari, Akhil
AU - Muraskas, Jonathan
PY - 2014/2
Y1 - 2014/2
N2 - Background Necrotizing enterocolitis (NEC) affects up to 10% of extremely-low-birthweight infants, with a 30% mortality rate. Currently, no biomarker reliably facilitates early diagnosis. Since thrombocytopenia and bowel ischemia are consistent findings in advanced NEC, we prospectively investigated two potential biomarkers: reticulated platelets (RP) and intestinal alkaline phosphatase (iAP). Methods Infants born ≤ 32 weeks and/or ≤ 1500 g were prospectively enrolled from 2009 to 2012. Starting within 72 hours of birth, 5 weekly whole blood specimens were collected to measure RP and serum iAP. Additional specimens were obtained at NEC onset (Bell stage II or III) and 24 hours later. Dichotomous cut-points were calculated for both biomarkers. Non-parametric (Mann-Whitney) and Chi-square tests were used to test differences between groups. Differences in Kaplan-Meier curves were examined by log-rank test. The Cox proportional hazards model estimated hazard ratios. Results A total of 177 infants were enrolled in the study, 15 (8.5%) of which developed NEC (40% required surgery and 20% died). 14 (93%) NEC infants had "low" (≤ 2.3%) reticulated platelets, and 9 (60%) had "high" iAP (> 0 U/L) in at least one sample before onset. Infants with "low" RP were significantly more likely to develop NEC [HR = 11.0 (1.4-83); P = 0.02]. Infants with "high" iAP were at increased risk for NEC, although not significant [HR = 5.2 (0.7-42); P = 0.12]. Median iAP levels were significantly higher at week 4 preceding the average time to NEC onset by one week (35.7 ± 17.3 days; P = 0.02). Conclusion Decreased RP serves as a sensitive marker for NEC onset, thereby enabling early preventative strategies. iAP overexpression may signal NEC development.
AB - Background Necrotizing enterocolitis (NEC) affects up to 10% of extremely-low-birthweight infants, with a 30% mortality rate. Currently, no biomarker reliably facilitates early diagnosis. Since thrombocytopenia and bowel ischemia are consistent findings in advanced NEC, we prospectively investigated two potential biomarkers: reticulated platelets (RP) and intestinal alkaline phosphatase (iAP). Methods Infants born ≤ 32 weeks and/or ≤ 1500 g were prospectively enrolled from 2009 to 2012. Starting within 72 hours of birth, 5 weekly whole blood specimens were collected to measure RP and serum iAP. Additional specimens were obtained at NEC onset (Bell stage II or III) and 24 hours later. Dichotomous cut-points were calculated for both biomarkers. Non-parametric (Mann-Whitney) and Chi-square tests were used to test differences between groups. Differences in Kaplan-Meier curves were examined by log-rank test. The Cox proportional hazards model estimated hazard ratios. Results A total of 177 infants were enrolled in the study, 15 (8.5%) of which developed NEC (40% required surgery and 20% died). 14 (93%) NEC infants had "low" (≤ 2.3%) reticulated platelets, and 9 (60%) had "high" iAP (> 0 U/L) in at least one sample before onset. Infants with "low" RP were significantly more likely to develop NEC [HR = 11.0 (1.4-83); P = 0.02]. Infants with "high" iAP were at increased risk for NEC, although not significant [HR = 5.2 (0.7-42); P = 0.12]. Median iAP levels were significantly higher at week 4 preceding the average time to NEC onset by one week (35.7 ± 17.3 days; P = 0.02). Conclusion Decreased RP serves as a sensitive marker for NEC onset, thereby enabling early preventative strategies. iAP overexpression may signal NEC development.
KW - Biomarker
KW - Extremely-low-birthweight
KW - Necrotizing enterocolitis
KW - Premature
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U2 - 10.1016/j.jpedsurg.2013.11.037
DO - 10.1016/j.jpedsurg.2013.11.037
M3 - Article
C2 - 24528965
AN - SCOPUS:84894070511
SN - 0022-3468
VL - 49
SP - 273
EP - 276
JO - Journal of pediatric surgery
JF - Journal of pediatric surgery
IS - 2
ER -