The relationship of single-strand breaks in DNA to breast cancer risk and to tissue concentrations of oestrogens

Mathavi Sahadevan, Oukseub Lee, Miguel Muzzio, Belinda Phan, Lisa Jacobs, Nagi Khouri, Jun Wang, Hong Hu, Vered Stearns, Robert T. Chatterton*

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Context: Clinical study of breast cancer patients in Chicago, IL, USA. Objective: Ascertain the utility of measurements of single-strand breaks (SSB) in DNA for assessment of breast cancer risk. Methods: Fine-needle aspirates of the breast, SSB by nick translation, percent breast density (PBD), Gail model risk, cumulative methylation index (CMI), enzymes of DNA repair and tissue antioxidants. Results: DNA repair enzymes and 4-hydroxyestradiol were negatively associated with SSB; CMI and PBD were positively associated. Conclusions: Quantitative measurement of SSBs by this procedure indicates the relative number of SSBs and is related to promoter methylation, antioxidant availability and percent breast density.

Original languageEnglish (US)
Pages (from-to)689-697
Number of pages9
JournalBiomarkers
Volume22
Issue number7
DOIs
StatePublished - Oct 3 2017

Fingerprint

Single-Stranded DNA Breaks
Methylation
DNA Repair Enzymes
Estrogens
Tissue
Breast Neoplasms
DNA
Antioxidants
Needles
Breast
Availability
Breast Density

Keywords

  • Breast cancer
  • DNA methylation
  • breast cancer risk
  • single-strand breaks in DNA
  • tissue oestrogens

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Health, Toxicology and Mutagenesis

Cite this

Sahadevan, Mathavi ; Lee, Oukseub ; Muzzio, Miguel ; Phan, Belinda ; Jacobs, Lisa ; Khouri, Nagi ; Wang, Jun ; Hu, Hong ; Stearns, Vered ; Chatterton, Robert T. / The relationship of single-strand breaks in DNA to breast cancer risk and to tissue concentrations of oestrogens. In: Biomarkers. 2017 ; Vol. 22, No. 7. pp. 689-697.
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The relationship of single-strand breaks in DNA to breast cancer risk and to tissue concentrations of oestrogens. / Sahadevan, Mathavi; Lee, Oukseub; Muzzio, Miguel; Phan, Belinda; Jacobs, Lisa; Khouri, Nagi; Wang, Jun; Hu, Hong; Stearns, Vered; Chatterton, Robert T.

In: Biomarkers, Vol. 22, No. 7, 03.10.2017, p. 689-697.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The relationship of single-strand breaks in DNA to breast cancer risk and to tissue concentrations of oestrogens

AU - Sahadevan, Mathavi

AU - Lee, Oukseub

AU - Muzzio, Miguel

AU - Phan, Belinda

AU - Jacobs, Lisa

AU - Khouri, Nagi

AU - Wang, Jun

AU - Hu, Hong

AU - Stearns, Vered

AU - Chatterton, Robert T.

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Y1 - 2017/10/3

N2 - Context: Clinical study of breast cancer patients in Chicago, IL, USA. Objective: Ascertain the utility of measurements of single-strand breaks (SSB) in DNA for assessment of breast cancer risk. Methods: Fine-needle aspirates of the breast, SSB by nick translation, percent breast density (PBD), Gail model risk, cumulative methylation index (CMI), enzymes of DNA repair and tissue antioxidants. Results: DNA repair enzymes and 4-hydroxyestradiol were negatively associated with SSB; CMI and PBD were positively associated. Conclusions: Quantitative measurement of SSBs by this procedure indicates the relative number of SSBs and is related to promoter methylation, antioxidant availability and percent breast density.

AB - Context: Clinical study of breast cancer patients in Chicago, IL, USA. Objective: Ascertain the utility of measurements of single-strand breaks (SSB) in DNA for assessment of breast cancer risk. Methods: Fine-needle aspirates of the breast, SSB by nick translation, percent breast density (PBD), Gail model risk, cumulative methylation index (CMI), enzymes of DNA repair and tissue antioxidants. Results: DNA repair enzymes and 4-hydroxyestradiol were negatively associated with SSB; CMI and PBD were positively associated. Conclusions: Quantitative measurement of SSBs by this procedure indicates the relative number of SSBs and is related to promoter methylation, antioxidant availability and percent breast density.

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KW - DNA methylation

KW - breast cancer risk

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KW - tissue oestrogens

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