TY - JOUR
T1 - The reorganization of microtubules and microfilaments in differentiating keratinocytes
AU - Lewis, Lindiann
AU - Barrandon, Yann
AU - Green, Howard
AU - Albrecht-Buehler, Guenter
PY - 1987
Y1 - 1987
N2 - Using immunofluorescence techniques, we have examined the microtubules and microfilaments in colonies of terminally differentiating human keratinocytes in tissue culture. The undifferentiated keratinocytes contained numerous microtubules, which radiated from a centrosomal organization center (MTOC). Differentiating keratinocytes, which leave the basal layer and begin to synthesize involucrin, displayed an altered cytoskeleton. Thick mats and coils of microtubules formed throughout the cytoplasm of the differentiated squames, and microfilaments were no longer visible after staining with phalloidin. Instead, only scattered stipples of phalloidin-stained material were observed. The results suggest that the terminal differentiation of epidermal cells involves a reorganization not only of the keratin filaments but of the entire cytoskeleton.
AB - Using immunofluorescence techniques, we have examined the microtubules and microfilaments in colonies of terminally differentiating human keratinocytes in tissue culture. The undifferentiated keratinocytes contained numerous microtubules, which radiated from a centrosomal organization center (MTOC). Differentiating keratinocytes, which leave the basal layer and begin to synthesize involucrin, displayed an altered cytoskeleton. Thick mats and coils of microtubules formed throughout the cytoplasm of the differentiated squames, and microfilaments were no longer visible after staining with phalloidin. Instead, only scattered stipples of phalloidin-stained material were observed. The results suggest that the terminal differentiation of epidermal cells involves a reorganization not only of the keratin filaments but of the entire cytoskeleton.
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U2 - 10.1111/j.1432-0436.1987.tb00197.x
DO - 10.1111/j.1432-0436.1987.tb00197.x
M3 - Article
C2 - 2452759
AN - SCOPUS:0011844545
SN - 0301-4681
VL - 36
SP - 228
EP - 233
JO - Differentiation
JF - Differentiation
IS - 3
ER -