The RNA binding region of the paramyxovirus SV5 V and P proteins

Grace Y. Lin, Reay G. Paterson, Robert A. Lamb*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

The V/P gene of simian virus 5 (SV5) encodes two proteins, V (222 residues) and the phosphoprotein P (392 residues). The V and P proteins are amino coterminal for 164 residues, but they have unique carboxy termini due to addition of two nontemplated G residues to the P mRNA during transcription. We have shown that the V and P proteins bind RNA by using both Northwestern blot analysis and ultraviolet-light crosslinking. The RNA- binding region has been mapped to a region in the P and V proteins which contains five basic residues (K74, K76, K77, R79, K81). Either deletion of the basic residues or substitution of the basic residues with alanine inhibited RNA binding by the V or P proteins.

Original languageEnglish (US)
Pages (from-to)460-469
Number of pages10
JournalVirology
Volume238
Issue number2
DOIs
StatePublished - Nov 24 1997

ASJC Scopus subject areas

  • Virology

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