Abstract
The V/P gene of simian virus 5 (SV5) encodes two proteins, V (222 residues) and the phosphoprotein P (392 residues). The V and P proteins are amino coterminal for 164 residues, but they have unique carboxy termini due to addition of two nontemplated G residues to the P mRNA during transcription. We have shown that the V and P proteins bind RNA by using both Northwestern blot analysis and ultraviolet-light crosslinking. The RNA- binding region has been mapped to a region in the P and V proteins which contains five basic residues (K74, K76, K77, R79, K81). Either deletion of the basic residues or substitution of the basic residues with alanine inhibited RNA binding by the V or P proteins.
Original language | English (US) |
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Pages (from-to) | 460-469 |
Number of pages | 10 |
Journal | Virology |
Volume | 238 |
Issue number | 2 |
DOIs | |
State | Published - Nov 24 1997 |
Funding
This work was supported by Research Grant AI-23173 from the National Institute of Allergy and Infectious Diseases. G.Y.L was supported by National Institutes of Health Medical Scientist Training Program Grant T32 GM-08152. R.A.L. is an Investigator of the Howard Hughes Medical Institute.
ASJC Scopus subject areas
- Virology