The testis-specific isozyme of lactate dehydrogenase, LDH-C4, provokes immune responses in SJL/J and C3H/HeJ mice that are regulated by an X-linked gene. To study control mechanisms that might be involved, crosses between these parental strains were made and followed by backcrosses of both (SJL x C3H) F1 and (C3H x SJL)F1 hybrids. The magnitude and kinetics of the antibody response to LDH-C4 by the backcross progeny were examined and the H-2 haplotypes were determined for each offspring. No H-2 control of the antibody response to this antigen could be demonstrated. Segregation of immune response classes was consistent with the existence of an X-linked gene involved in the regulation of the secondary humoral response kinetics. This interpretation was strongly supported by results showing that the kinetics of the immune response were independent of antigen dosage in all parental and F1 strain and sex classes. Antiserum samples from parental and F1 hybrid strains were also analyzed for their relative concentration of IgM and IgG class antibody. Total anti-LDH-C4 antibody concentrations and relative amounts of anti-LDH-C4 IgM and IgG were measured in a solid-phase radioimmune assay. The kinetics of responsiveness determined in this way were in good agreement with previous studies but no class differences were found in the anti-LDH-C4 antibody of the different strains and hybrids. Finally, observations were made on sex-related differences and possible maternal effects that are manifest as variations in the magnitude of the humoral response to the LDH-C4 antigen.
|Original language||English (US)|
|Number of pages||7|
|Journal||Journal of Immunology|
|State||Published - Jan 1 1981|
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