The role of internalization in transforming growth factor β1-induced Smad2 association with Smad anchor for receptor activation (SARA) and Smad2-dependent signaling in human mesangial cells

Constance E. Runyan*, H. William Schnaper, Anne Christine Poncelet

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

92 Scopus citations

Abstract

Recent data investigating the role of the Smad anchor for receptor activation (SARA) in TGF-β signaling have suggested that it has a crucial function in both aiding the recruitment of Smad to the TGF-β receptor, and ensuring appropriate subcellular localization of the activated receptor-bound complex. The FYVE domain in SARA directs its localization to early endosomal compartments where it can interact with both the TGF-β receptors and Smads. However, the necessity of endocytosis in the TGF-β response remains controversial. We sought to examine the role of internalization in TGF-β/ Smad signaling in human kidney mesangial cells. Using co-immunoprecipitation studies, we show that endogenous Smad2 interacts with SARA after TGF-β1 stimulation. Inhibition of clathrin-mediated internalization only slightly affects TGF-β1-stimulated association between SARA and Smad2, Smad2 phosphorylation, or Smad2 interaction with Smad4. However, endocytosis inhibition decreases TGF-β1-induced Smad2 nuclear translocation and thus abrogates Smad2-dependent transcriptional responses. The TGF-β1-stimulated association between SARA and Smad2 peaks at 30 min followed by separation of the complex components. However, under conditions of inhibited endocytosis, Smad2 remains bound to SARA for at least 6 h without a significant decline in associated levels. This lack of complex dissociation correlates with a lack of Smad2 nuclear accumulation and reduction of 3mad2-dependent ARE-Luc reporter activity. Our data therefore suggest that endocytosis plays a critical role in TGF-β signaling in mesangial cells, and that internalization enhances the dissociation of Smad2 from the TGF-β receptor-SARA complex, allowing Smad2 to accumulate in the nucleus and modulate target gene transcription.

Original languageEnglish (US)
Pages (from-to)8300-8308
Number of pages9
JournalJournal of Biological Chemistry
Volume280
Issue number9
DOIs
StatePublished - Mar 4 2005

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'The role of internalization in transforming growth factor β1-induced Smad2 association with Smad anchor for receptor activation (SARA) and Smad2-dependent signaling in human mesangial cells'. Together they form a unique fingerprint.

Cite this