The biological activity of immobilized carbohydrates can show a dramatic dependence on the density of carbohydrate. This is the result of investigations with self-assembled monolayers that present N- acetylglucosamine groups as a model substrate for glycosylation by bovine β- 1,4-galactosyltransferase (GalTase; see picture). Surface plasmon resonance spectroscopy and carbohydrate-binding lectins were used to characterize the reaction at the interface. UDP-Gal = uridine diphosphogalactose.
|Original language||English (US)|
|Number of pages||4|
|Journal||Angewandte Chemie - International Edition|
|State||Published - Mar 15 1999|
- Interfaces monolayers
- Surface plasmon resonance spectroscopy
ASJC Scopus subject areas