The role of PECAM-1 (CD31) in leukocyte emigration: Studies in vitro and in vivo

W. A. Muller*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

172 Scopus citations

Abstract

Platelet/endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a molecule capable of mediating both hemophilic and heterophilic adhesion. It is constitutively expressed and concentrated in the lateral borders between endothelial cells and expressed on the surfaces of neutrophils, monocytes, and some T cell subsets, as well as on platelets. In a quantitative in vitro assay, monoclonal antibody against PECAM-1 or soluble recombinant PECAM-1 selectively blocked passage of both neutrophils and monocytes across the endothelial monolayer by 70-90% without interfering with the ability of these cells to bind to the apical endothelial cell surface. These reagents worked whether directed against leukocyte PECAM-1 or against endothelial cell PECAM-1 and were not additive, suggesting that a hemophilic interaction was occurring. In a murine mode! of acute inflammation, thioglycollate-induced peritonitis, a monoclonal antibody against mouse PECAM-1 blocked emigration of leukocytes into the peritoneal cavity down to background levels. Examination of peritoneal venules in these mice revealed many leukocytes in apparent contact with the endothelial surface but unable to cross the intima. Thus, PECAM-1. has a distinct role in the transendothelial migration phase of leukocyte emigration, independent of the adhesion events on the apical surface.

Original languageEnglish (US)
Pages (from-to)523-528
Number of pages6
JournalJournal of Leukocyte Biology
Volume57
Issue number4
DOIs
StatePublished - 1995

Keywords

  • Adhesion molecule
  • Endothelial cells
  • Monocyte
  • Neutrophil
  • Platelet/endothelia1 cell adhesion molecule 1
  • Transendothelial migration

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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