TY - JOUR
T1 - The role of stem cell factor and granulocyte-colony stimulating factor in brain repair during chronic stroke
AU - Piao, Chun Shu
AU - Gonzalez-Toledo, Maria E.
AU - Xue, Yue Qiang
AU - Duan, Wei Ming
AU - Terao, Satoshi
AU - Granger, D. Neil
AU - Kelley, Roger E.
AU - Zhao, Li Ru
PY - 2009/4
Y1 - 2009/4
N2 - Chronic stroke is a highly important but under-investigated scientific problem in neurologic research. We have reported earlier that stem cell factor (SCF) in combination with granulocyte-colony stimulating factor (G-CSF) treatment during chronic stroke improves functional outcomes. Here we have determined the contribution of bone marrow-derived cells in angiogenesis and neurogenesis, which are enhanced by SCFG-CSF treatment during chronic stroke. Using bone marrow tracking, flow cytometry, 2-photon live brain imaging, and immunohistochemistry, we observed that the levels of circulating bone marrow stem cells (BMSCs) (CD34 /c-kit ) were significantly increased by SCFG-CSF treatment. In addition, live brain imaging revealed that numerous bone marrow-derived cells migrate into the brain parenchyma in the treated mice. We also found that bone marrow-derived cells, bone marrow-derived endothelial cells, vascular density, and bone marrow-derived neurons were significantly augmented by SCFG-CSF. It is interesting that, in addition to the increase in bone marrow-derived endothelial cells, the number of bone marrow-derived pericytes was reduced after SCFG-CSF treatment during chronic stroke. These data suggest that SCFG-CSF treatment can enhance repair of brain damage during chronic stroke by mobilizing BMSCs, and promoting the contribution of bone marrow-derived cells to angiogenesis and neurogenesis.
AB - Chronic stroke is a highly important but under-investigated scientific problem in neurologic research. We have reported earlier that stem cell factor (SCF) in combination with granulocyte-colony stimulating factor (G-CSF) treatment during chronic stroke improves functional outcomes. Here we have determined the contribution of bone marrow-derived cells in angiogenesis and neurogenesis, which are enhanced by SCFG-CSF treatment during chronic stroke. Using bone marrow tracking, flow cytometry, 2-photon live brain imaging, and immunohistochemistry, we observed that the levels of circulating bone marrow stem cells (BMSCs) (CD34 /c-kit ) were significantly increased by SCFG-CSF treatment. In addition, live brain imaging revealed that numerous bone marrow-derived cells migrate into the brain parenchyma in the treated mice. We also found that bone marrow-derived cells, bone marrow-derived endothelial cells, vascular density, and bone marrow-derived neurons were significantly augmented by SCFG-CSF. It is interesting that, in addition to the increase in bone marrow-derived endothelial cells, the number of bone marrow-derived pericytes was reduced after SCFG-CSF treatment during chronic stroke. These data suggest that SCFG-CSF treatment can enhance repair of brain damage during chronic stroke by mobilizing BMSCs, and promoting the contribution of bone marrow-derived cells to angiogenesis and neurogenesis.
KW - Angiogenesis
KW - Brain imaging
KW - Chronic stroke
KW - Hematopoietic growth factors
UR - http://www.scopus.com/inward/record.url?scp=63949087101&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=63949087101&partnerID=8YFLogxK
U2 - 10.1038/jcbfm.2008.168
DO - 10.1038/jcbfm.2008.168
M3 - Article
C2 - 19209180
AN - SCOPUS:63949087101
VL - 29
SP - 759
EP - 770
JO - Journal of Cerebral Blood Flow and Metabolism
JF - Journal of Cerebral Blood Flow and Metabolism
SN - 0271-678X
IS - 4
ER -