The role of stem cell factor and granulocyte-colony stimulating factor in brain repair during chronic stroke

Chun Shu Piao, Maria E. Gonzalez-Toledo, Yue Qiang Xue, Wei Ming Duan, Satoshi Terao, D. Neil Granger, Roger E. Kelley, Li Ru Zhao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Chronic stroke is a highly important but under-investigated scientific problem in neurologic research. We have reported earlier that stem cell factor (SCF) in combination with granulocyte-colony stimulating factor (G-CSF) treatment during chronic stroke improves functional outcomes. Here we have determined the contribution of bone marrow-derived cells in angiogenesis and neurogenesis, which are enhanced by SCFG-CSF treatment during chronic stroke. Using bone marrow tracking, flow cytometry, 2-photon live brain imaging, and immunohistochemistry, we observed that the levels of circulating bone marrow stem cells (BMSCs) (CD34 /c-kit ) were significantly increased by SCFG-CSF treatment. In addition, live brain imaging revealed that numerous bone marrow-derived cells migrate into the brain parenchyma in the treated mice. We also found that bone marrow-derived cells, bone marrow-derived endothelial cells, vascular density, and bone marrow-derived neurons were significantly augmented by SCFG-CSF. It is interesting that, in addition to the increase in bone marrow-derived endothelial cells, the number of bone marrow-derived pericytes was reduced after SCFG-CSF treatment during chronic stroke. These data suggest that SCFG-CSF treatment can enhance repair of brain damage during chronic stroke by mobilizing BMSCs, and promoting the contribution of bone marrow-derived cells to angiogenesis and neurogenesis.

Original languageEnglish (US)
Pages (from-to)759-770
Number of pages12
JournalJournal of Cerebral Blood Flow and Metabolism
Volume29
Issue number4
DOIs
StatePublished - Apr 2009

Keywords

  • Angiogenesis
  • Brain imaging
  • Chronic stroke
  • Hematopoietic growth factors

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

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