The Role of Transcription Factors and Nuclear Pore Proteins in Controlling the Spatial Organization of the Yeast Genome

Donna Garvey Brickner, Carlo Randise-Hinchliff, Marine Lebrun Corbin, Julie Ming Liang, Stephanie Kim, Bethany Sump, Agustina D'Urso, Seo Hyun Kim, Atsushi Satomura, Heidi Schmit, Robert Coukos, Subin Hwang, Raven Watson, Jason H. Brickner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Loss of nuclear pore complex (NPC) proteins, transcription factors (TFs), histone modification enzymes, Mediator, and factors involved in mRNA export disrupts the physical interaction of chromosomal sites with NPCs. Conditional inactivation and ectopic tethering experiments support a direct role for the TFs Gcn4 and Nup2 in mediating interaction with the NPC but suggest an indirect role for factors involved in mRNA export or transcription. A conserved “positioning domain” within Gcn4 controls interaction with the NPC and inter-chromosomal clustering and promotes transcription of target genes. Such a function may be quite common; a comprehensive screen reveals that tethering of most yeast TFs is sufficient to promote targeting to the NPC. While some TFs require Nup100, others do not, suggesting two distinct targeting mechanisms. These results highlight an important and underappreciated function of TFs in controlling the spatial organization of the yeast genome through interaction with the NPC. Brickner et al. show that transcription factors (TFs) control positioning of genes through interaction with the nuclear pore complex. A “positioning domain” from one TF promotes interaction with the pore, and a global screen reveals that most yeast TFs mediate targeting to the NPC using at least two different pathways.

Original languageEnglish (US)
Pages (from-to)936-947.e4
JournalDevelopmental Cell
Volume49
Issue number6
DOIs
StatePublished - Jun 17 2019

Keywords

  • chromatin
  • chromosome
  • inter-chromosomal clustering
  • nuclear architecture
  • nuclear pore complex
  • transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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