@article{cb16db0869f447bbb1bbead19b84ff0b,
title = "The Role of Transcription Factors and Nuclear Pore Proteins in Controlling the Spatial Organization of the Yeast Genome",
abstract = "Loss of nuclear pore complex (NPC) proteins, transcription factors (TFs), histone modification enzymes, Mediator, and factors involved in mRNA export disrupts the physical interaction of chromosomal sites with NPCs. Conditional inactivation and ectopic tethering experiments support a direct role for the TFs Gcn4 and Nup2 in mediating interaction with the NPC but suggest an indirect role for factors involved in mRNA export or transcription. A conserved “positioning domain” within Gcn4 controls interaction with the NPC and inter-chromosomal clustering and promotes transcription of target genes. Such a function may be quite common; a comprehensive screen reveals that tethering of most yeast TFs is sufficient to promote targeting to the NPC. While some TFs require Nup100, others do not, suggesting two distinct targeting mechanisms. These results highlight an important and underappreciated function of TFs in controlling the spatial organization of the yeast genome through interaction with the NPC. Brickner et al. show that transcription factors (TFs) control positioning of genes through interaction with the nuclear pore complex. A “positioning domain” from one TF promotes interaction with the pore, and a global screen reveals that most yeast TFs mediate targeting to the NPC using at least two different pathways.",
keywords = "chromatin, chromosome, inter-chromosomal clustering, nuclear architecture, nuclear pore complex, transcription",
author = "Brickner, {Donna Garvey} and Carlo Randise-Hinchliff and Corbin, {Marine Lebrun} and Liang, {Julie Ming} and Stephanie Kim and Bethany Sump and Agustina D'Urso and Kim, {Seo Hyun} and Atsushi Satomura and Heidi Schmit and Robert Coukos and Subin Hwang and Raven Watson and Brickner, {Jason H.}",
note = "Funding Information: The authors thank members of the Brickner laboratory, Erik Andersen, and Alan Hinnebusch for helpful discussions and input. This work was supported by NIH R01 GM 080484 and R01 GM118712 (J.H.B.). A.D. was supported by NIH T32 GM008061; R.C. S.K. S.H.K. S.H. and R.W. were supported by Northwestern University undergraduate research fellowships. A.S. is supported by a JSPS Overseas postdoctoral fellowship from the Japanese Society for the Promotion of Science. Conceptualization, J.H.B. D.G.B. and C.R.H.; Methodology, D.G.B. C.R.H. and J.M.L.; Software and Formal Statistical Analysis, J.H.B. and A.S.; Investigation, D.G.B. C.R.H. M.L.C. J.M.L. S.K. B.S. A.D. S.H.K. H.S. R.C. S.H. and R.W.; Writing, J.H.B. D.G.B. and C.R.H.; Visualization, J.H.B. D.G.B. and C.R.H.; Supervision, J.H.B. and D.G.B.; Project Administration, J.H.B.; Funding Acquisition, J.H.B. The authors declare no competing interests. Funding Information: The authors thank members of the Brickner laboratory, Erik Andersen, and Alan Hinnebusch for helpful discussions and input. This work was supported by NIH R01 GM 080484 and R01 GM118712 (J.H.B.). A.D. was supported by NIH T32 GM008061 ; R.C., S.K., S.H.K., S.H., and R.W. were supported by Northwestern University undergraduate research fellowships. A.S. is supported by a JSPS Overseas postdoctoral fellowship from the Japanese Society for the Promotion of Science. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = jun,
day = "17",
doi = "10.1016/j.devcel.2019.05.023",
language = "English (US)",
volume = "49",
pages = "936--947.e4",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "6",
}