The Self-Splicing RNA of Tetrahymena Is Trapped in a Less Active Conformation by Gel Purification

S. A. Walstrum, O. C. Uhlenbeck*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

When the circular form of the self-splicing intervening sequence of Tetrahymena thermophila was purified by denaturing polyacrylamide gel electrophoresis by standard methods, the rate of its reaction with tetrauridylate decreased 150-fold at 30 °C and at least 1000-fold at 0 °C. The activity of the self-splicing RNA was restored by heating it to high temperature and letting it renature in the presence of Mg2+. The rate of reaction of tetrauridylate with the self-splicing RNA flanked by exons was also greatly decreased by gel purification. the difference in activation energies for the reaction of native and denatured intervening sequences suggests that a substantial conformational rearrangement of the gel-purified RNA occurs prior to reaction.

Original languageEnglish (US)
Pages (from-to)10573-10576
Number of pages4
JournalBiochemistry
Volume29
Issue number46
DOIs
StatePublished - Nov 1 1990

ASJC Scopus subject areas

  • Biochemistry

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