The small molecule AMBMP disrupts microtubule growth, ciliogenesis, cell polarity, and cell migration

Michael Werner, Urko del Castillo, Rosa Ventrella, Eva Brotslaw, Brian Mitchell*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

2-Amino-4-(3,4-[methylenedioxy]benzylamino)-6-(3-methoxyphenyl)pyrimidine (AMBMP) is a small molecule that has been previously reported to be both a Wnt agonist and a microtubule (MT) regulator. Here we report a detailed analysis of AMBMPs effects on MTs and on MT associated cellular processes including cell polarity, ciliogenesis, and cell migration. Specifically, treatment of Xenopus embryos with AMBMP leads to defects similar to the MT depolymerizing drug nocodazole, including a failure to generate or polarize cilia (depending on the timing of treatment) and a loss of the cell movements associated with radial intercalation. The dramatic effect AMBMP has on basic MT based cellular functions suggests that its usefulness as a Wnt regulator is questionable. Moreover, it may be an important new tool for experimental or pharmacological manipulation of MTs.

Original languageEnglish (US)
Pages (from-to)450-457
Number of pages8
JournalCytoskeleton
Volume75
Issue number10
DOIs
StatePublished - Oct 2018

Keywords

  • Wnt signaling
  • cell polarity
  • ciliogenesis
  • microtubule
  • small molecule

ASJC Scopus subject areas

  • Structural Biology
  • Cell Biology

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