The sphingosine kinase 1 inhibitor, pf543, mitigates pulmonary fibrosis by reducing lung epithelial cell mtdna damage and recruitment of fibrogenic monocytes

Paul Cheresh, Seok Jo Kim, Long Shuang Huang, Satoshi Watanabe, Nikita Joshi, Kinola J.N. Williams, Monica Chi, Ziyan Lu, Anantha Harijith, Anjana Yeldandi, Anna P. Lam, Cara Gottardi, Alexander V. Misharin, G. R.Scott Budinger, Viswanathan Natarajan, David W. Kamp*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Idiopathic pulmonary fibrosis (IPF) is a chronic disease for which novel approaches are urgently required. We reported increased sphingosine kinase 1 (SPHK1) in IPF lungs and that SPHK1 inhibition using genetic and pharmacologic approaches reduces murine bleomycin-induced pulmonary fibrosis. We determined whether PF543, a specific SPHK1 inhibitor post bleomycin or asbestos challenge mitigates lung fibrosis by reducing mitochondrial (mt) DNA damage and pro-fibrotic monocyte recruitment—both are implicated in the pathobiology of pulmonary fibrosis. Bleomycin (1.5 U/kg), crocidolite asbestos (100 µg/50 µL) or controls was intratracheally instilled in Wild-Type (C57Bl6) mice. PF543 (1 mg/kg) or vehicle was intraperitoneally injected once every two days from day 7−21 following bleomycin and day 14−21 or day 30−60 following asbestos. PF543 reduced bleomycin-and asbestos-induced pulmonary fibrosis at both time points as well as lung expression of profibrotic markers, lung mtDNA damage, and fibrogenic monocyte recruitment. In contrast to human lung fibroblasts, asbestos augmented lung epithelial cell (MLE) mtDNA damage and PF543 was protective. Post-exposure PF543 mitigates pulmonary fibrosis in part by reducing lung epithelial cell mtDNA damage and monocyte recruitment. We reason that SPHK1 signaling may be an innovative therapeutic target for managing patients with IPF and other forms of lung fibrosis.

Original languageEnglish (US)
Article number5595
Pages (from-to)1-17
Number of pages17
JournalInternational journal of molecular sciences
Volume21
Issue number16
DOIs
StatePublished - 2020

Keywords

  • Alveolar epithelial cell
  • Monocytes
  • MtDNA damage
  • Oxidative stress
  • Pulmonary fibrosis
  • SPHK1

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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