The tetrahydrobiopterin radical interacting with high- and low-spin heme in neuronal nitric oxide synthase – A new indicator of the extent of NOS coupling

Matthew D Krzyaniak, Alex A. Cruce, Preethi Vennam, Molly Lockart, Vladimir Berka, Ah Lim Tsai, Michael K. Bowman*

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Reaction intermediates trapped during the single-turnover reaction of the neuronal ferrous nitric oxide synthase oxygenase domain (Fe(II)nNOSOX) show four EPR spectra of free radicals. Fully-coupled nNOSOX with cofactor (tetrahydrobiopterin, BH4) and substrate (L-arginine) forms the typical BH4 cation radical with an EPR spectrum ~4.0 mT wide and hyperfine tensors similar to reports for a biopterin cation radical in inducible NOSOX (iNOSOX). With excess thiol, nNOSox lacking BH4 and L-arg is known to produce superoxide. In contrast, we find that nNOSOX with BH4 but no L-arg forms two radicals with rather different, fast (~250 μs at 5 K) and slower (~500 μs at 20 K), electron spin relaxation rates and a combined ~7.0 mT wide EPR spectrum. Rapid freeze-quench CW- and pulsed-EPR measurements are used to identify these radicals and their origin. These two species are the same radical with identical nuclear hyperfine couplings, but with spin-spin couplings to high-spin (4.0 mT component) or low-spin (7.0 mT component) Fe(III) heme. Uncoupled reactions of nNOS leave the enzyme in states that can be chemically reduced to sustain unregulated production of NO and reactive oxygen species in ischemia-reperfusion injury. The broad EPR signal is a convenient indicator of uncoupled nNOS reactions producing low-spin Fe(III) heme.

Original languageEnglish (US)
Pages (from-to)367-377
Number of pages11
JournalFree Radical Biology and Medicine
Volume101
DOIs
StatePublished - Dec 1 2016

Keywords

  • ENDOR
  • EPR
  • ESEEM
  • Nitric oxide synthase
  • Tetrahydrobiopterin radical

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

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