The Tetrahymena telomerase p75-p45-p19 subcomplex is a unique CST complex

Bingbing Wan, Ting Tang, Heather Upton, Jin Shuai, Yuanzhe Zhou, Song Li, Juan Chen, Joseph S. Brunzelle, Zhixiong Zeng, Kathleen Collins, Jian Wu*, Ming Lei

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    25 Scopus citations

    Abstract

    Tetrahymena telomerase holoenzyme subunits p75, p45 and p19 form a subcomplex (7-4-1) peripheral to the catalytic core. We report structures of p45 and p19 and reveal them as the Stn1 and Ten1 subunits of the CST complex, which stimulates telomerase complementary-strand synthesis. 7-4-1 binds telomeric single-stranded DNA, and mutant p19 overexpression causes telomere 3′-overhang elongation. We propose that telomerase-tethered Tetrahymena CST coordinates telomere G-strand and C-strand synthesis.

    Original languageEnglish (US)
    Pages (from-to)1023-1026
    Number of pages4
    JournalNature Structural and Molecular Biology
    Volume22
    Issue number12
    DOIs
    StatePublished - Dec 1 2015

    ASJC Scopus subject areas

    • Structural Biology
    • Molecular Biology

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