The Tetrahymena telomerase p75-p45-p19 subcomplex is a unique CST complex

Bingbing Wan; Ting Tang; Heather Upton; Jin Shuai; Yuanzhe Zhou; Song Li; Juan Chen; Joseph S. Brunzelle; Zhixiong Zeng; Kathleen Collins; Jian Wu; Ming Lei

doi:10.1038/nsmb.3126

The Tetrahymena telomerase p75-p45-p19 subcomplex is a unique CST complex

Bingbing Wan, Ting Tang, Heather Upton, Jin Shuai, Yuanzhe Zhou, Song Li, Juan Chen, Joseph S. Brunzelle, Zhixiong Zeng, Kathleen Collins, Jian Wu^*, Ming Lei

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Tetrahymena telomerase holoenzyme subunits p75, p45 and p19 form a subcomplex (7-4-1) peripheral to the catalytic core. We report structures of p45 and p19 and reveal them as the Stn1 and Ten1 subunits of the CST complex, which stimulates telomerase complementary-strand synthesis. 7-4-1 binds telomeric single-stranded DNA, and mutant p19 overexpression causes telomere 3′-overhang elongation. We propose that telomerase-tethered Tetrahymena CST coordinates telomere G-strand and C-strand synthesis.

Original language	English (US)
Pages (from-to)	1023-1026
Number of pages	4
Journal	Nature Structural and Molecular Biology
Volume	22
Issue number	12
DOIs	https://doi.org/10.1038/nsmb.3126
State	Published - Dec 1 2015

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1038/nsmb.3126

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abstract = "Tetrahymena telomerase holoenzyme subunits p75, p45 and p19 form a subcomplex (7-4-1) peripheral to the catalytic core. We report structures of p45 and p19 and reveal them as the Stn1 and Ten1 subunits of the CST complex, which stimulates telomerase complementary-strand synthesis. 7-4-1 binds telomeric single-stranded DNA, and mutant p19 overexpression causes telomere 3′-overhang elongation. We propose that telomerase-tethered Tetrahymena CST coordinates telomere G-strand and C-strand synthesis.",

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AU - Wan, Bingbing

AU - Tang, Ting

AU - Upton, Heather

AU - Shuai, Jin

AU - Zhou, Yuanzhe

AU - Li, Song

AU - Chen, Juan

AU - Brunzelle, Joseph S.

AU - Zeng, Zhixiong

AU - Collins, Kathleen

AU - Wu, Jian

AU - Lei, Ming

PY - 2015/12/1

Y1 - 2015/12/1

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AB - Tetrahymena telomerase holoenzyme subunits p75, p45 and p19 form a subcomplex (7-4-1) peripheral to the catalytic core. We report structures of p45 and p19 and reveal them as the Stn1 and Ten1 subunits of the CST complex, which stimulates telomerase complementary-strand synthesis. 7-4-1 binds telomeric single-stranded DNA, and mutant p19 overexpression causes telomere 3′-overhang elongation. We propose that telomerase-tethered Tetrahymena CST coordinates telomere G-strand and C-strand synthesis.

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The Tetrahymena telomerase p75-p45-p19 subcomplex is a unique CST complex

Abstract

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Crystal structure of Tetrahymena p45C

Crystal structure of Tetrahymena p19

Crystal structure of Tetrahymena p45N and p19

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The Tetrahymena telomerase p75-p45-p19 subcomplex is a unique CST complex

Abstract

ASJC Scopus subject areas

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Datasets

Crystal structure of Tetrahymena p45C

Crystal structure of Tetrahymena p19

Crystal structure of Tetrahymena p45N and p19

Cite this