The transient responses of hybridoma cells to nutrient additions in continuous culture: II. Glutamine pulse and step changes

William M Miller*, Charles R. Wilke, Harvey W. Blanch

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

The transient and steady‐state responses of hybridoma growth and metabolism to glutamine pulse and step changes have been examined. Metabolic quotients are reported for oxygen, glucose, lactate, ammonia, glutamine, alanine, and other amino acids. The specific glutamine consumption rate increased rapidly after all glutamine additions, but the responses of the glucose and oxygen consumption rates and the cell concentration were found to depend on the intial feed glutamine concentration. The glucose consumption rate was 1.4‐10.9 times that of glutamine, and serine and branched‐chain amino acids were consumed in larger amounts at the higher glucose: glutamine uptake ratios. It was estimated that maintenance accounted for ca. 60% of the cellular ATP requirements at specific growth rates ranging from 0.57 to 0.68 day−1.

Original languageEnglish (US)
Pages (from-to)487-499
Number of pages13
JournalBiotechnology and Bioengineering
Volume33
Issue number4
DOIs
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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