The trithorax-group gene in Drosophila little imaginal discs encodes a trimethylated histone H3 Lys4 demethylase

Joel C. Eissenberg; Min Gyu Lee; Jessica Schneider; Anne Ilvarsonn; Ramin Shiekhattar; Ali Shilatifard

doi:10.1038/nsmb1217

The trithorax-group gene in Drosophila little imaginal discs encodes a trimethylated histone H3 Lys4 demethylase

Joel C. Eissenberg, Min Gyu Lee, Jessica Schneider, Anne Ilvarsonn, Ramin Shiekhattar, Ali Shilatifard^*

^*Corresponding author for this work

Biochemistry and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

97 Scopus citations

Abstract

Histone H3 Lys4 (H3K4) is methylated by yeast Set1-COMPASS and its mammalian homolog, the MLL complex. Human JARID1d can demethylate trimethyl-H3K4 (H3K4me3). We identified Drosophila melanogaster little imaginal discs (Lid) as the JARID1d homolog. We report that Lid knockdown using RNA interference results in a specific genome-wide increase in H3K4me3 levels without affecting other patterns of H3 methylation, and results in an altered distribution of the chromo-helicase protein Chd1.

Original language	English (US)
Pages (from-to)	344-346
Number of pages	3
Journal	Nature Structural and Molecular Biology
Volume	14
Issue number	4
DOIs	https://doi.org/10.1038/nsmb1217
State	Published - Apr 2007

Funding

We thank R. Perry (Fox Chase Cancer Center) for the Chd1 antiserum. J.C.E. was supported by grants from the US National Science Foundation (MCB 0131414 and MCB 0615831). R.S. was supported by a grant from the US National Institutes of Health (GM61204). A.S. was supported by grants from the US National Institutes of Health (CA089455 and GM069905).

ASJC Scopus subject areas

Molecular Biology
Structural Biology

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title = "The trithorax-group gene in Drosophila little imaginal discs encodes a trimethylated histone H3 Lys4 demethylase",

abstract = "Histone H3 Lys4 (H3K4) is methylated by yeast Set1-COMPASS and its mammalian homolog, the MLL complex. Human JARID1d can demethylate trimethyl-H3K4 (H3K4me3). We identified Drosophila melanogaster little imaginal discs (Lid) as the JARID1d homolog. We report that Lid knockdown using RNA interference results in a specific genome-wide increase in H3K4me3 levels without affecting other patterns of H3 methylation, and results in an altered distribution of the chromo-helicase protein Chd1.",

author = "Eissenberg, {Joel C.} and Lee, {Min Gyu} and Jessica Schneider and Anne Ilvarsonn and Ramin Shiekhattar and Ali Shilatifard",

note = "Funding Information: We thank R. Perry (Fox Chase Cancer Center) for the Chd1 antiserum. J.C.E. was supported by grants from the US National Science Foundation (MCB 0131414 and MCB 0615831). R.S. was supported by a grant from the US National Institutes of Health (GM61204). A.S. was supported by grants from the US National Institutes of Health (CA089455 and GM069905).",

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AU - Schneider, Jessica

AU - Ilvarsonn, Anne

AU - Shiekhattar, Ramin

AU - Shilatifard, Ali

N1 - Funding Information: We thank R. Perry (Fox Chase Cancer Center) for the Chd1 antiserum. J.C.E. was supported by grants from the US National Science Foundation (MCB 0131414 and MCB 0615831). R.S. was supported by a grant from the US National Institutes of Health (GM61204). A.S. was supported by grants from the US National Institutes of Health (CA089455 and GM069905).

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N2 - Histone H3 Lys4 (H3K4) is methylated by yeast Set1-COMPASS and its mammalian homolog, the MLL complex. Human JARID1d can demethylate trimethyl-H3K4 (H3K4me3). We identified Drosophila melanogaster little imaginal discs (Lid) as the JARID1d homolog. We report that Lid knockdown using RNA interference results in a specific genome-wide increase in H3K4me3 levels without affecting other patterns of H3 methylation, and results in an altered distribution of the chromo-helicase protein Chd1.

AB - Histone H3 Lys4 (H3K4) is methylated by yeast Set1-COMPASS and its mammalian homolog, the MLL complex. Human JARID1d can demethylate trimethyl-H3K4 (H3K4me3). We identified Drosophila melanogaster little imaginal discs (Lid) as the JARID1d homolog. We report that Lid knockdown using RNA interference results in a specific genome-wide increase in H3K4me3 levels without affecting other patterns of H3 methylation, and results in an altered distribution of the chromo-helicase protein Chd1.

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The trithorax-group gene in Drosophila little imaginal discs encodes a trimethylated histone H3 Lys4 demethylase

Abstract

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