Abstract
Uropathogenic Escherichia coli (UPEC) fall within a larger group of isolates producing extraintestinal disease. UPEC express type 1 pili as a critical virulence determinant mediating adherence to and invasion into urinary tract tissues. Type 1 pili expression is under regulation by a family of site-specific recombinases, including FimX, which is encoded from a genomic island called PAI-X for pathogenicity island of FimX. Using a new multiplex PCR, fimX and the additional PAI-X genes were found to be highly associated with UPEC (144/173583.2 %), and more prevalent in UPEC of lower urinary tract origin (105/120587.5 %) than upper urinary tract origin (39/53574 %; P,0.05) or commensal isolates (28/78536 %; P¡0.0001). The Fim-like recombinase gene fimX is the only family member that has a significant association with UPEC compared to commensal isolates. Our results indicate PAI-X genes, including the type 1 pili regulator gene fimX, are highly prevalent among UPEC isolates and have a strong positive correlation with genomic virulence factors, suggesting a potential role for PAI-X in the extraintestinal pathogenic E. coli lifestyle.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1606-1617 |
| Number of pages | 12 |
| Journal | Microbiology (United Kingdom) |
| Volume | 159 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 1 2013 |
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ASJC Scopus subject areas
- Microbiology
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The type 1 pili regulator gene fimX and pathogenicity island PAI-X as molecular markers of uropathogenic Escherichia coli. / Bateman, Stacey L.; Stapleton, Ann E.; Stamm, Walter E.; Hooton, Thomas M.; Seed, Patrick C.
In: Microbiology (United Kingdom), Vol. 159, No. 8, 01.08.2013, p. 1606-1617.Research output: Contribution to journal › Article
TY - JOUR
T1 - The type 1 pili regulator gene fimX and pathogenicity island PAI-X as molecular markers of uropathogenic Escherichia coli
AU - Bateman, Stacey L.
AU - Stapleton, Ann E.
AU - Stamm, Walter E.
AU - Hooton, Thomas M.
AU - Seed, Patrick C.
PY - 2013/8/1
Y1 - 2013/8/1
N2 - Uropathogenic Escherichia coli (UPEC) fall within a larger group of isolates producing extraintestinal disease. UPEC express type 1 pili as a critical virulence determinant mediating adherence to and invasion into urinary tract tissues. Type 1 pili expression is under regulation by a family of site-specific recombinases, including FimX, which is encoded from a genomic island called PAI-X for pathogenicity island of FimX. Using a new multiplex PCR, fimX and the additional PAI-X genes were found to be highly associated with UPEC (144/173583.2 %), and more prevalent in UPEC of lower urinary tract origin (105/120587.5 %) than upper urinary tract origin (39/53574 %; P,0.05) or commensal isolates (28/78536 %; P¡0.0001). The Fim-like recombinase gene fimX is the only family member that has a significant association with UPEC compared to commensal isolates. Our results indicate PAI-X genes, including the type 1 pili regulator gene fimX, are highly prevalent among UPEC isolates and have a strong positive correlation with genomic virulence factors, suggesting a potential role for PAI-X in the extraintestinal pathogenic E. coli lifestyle.
AB - Uropathogenic Escherichia coli (UPEC) fall within a larger group of isolates producing extraintestinal disease. UPEC express type 1 pili as a critical virulence determinant mediating adherence to and invasion into urinary tract tissues. Type 1 pili expression is under regulation by a family of site-specific recombinases, including FimX, which is encoded from a genomic island called PAI-X for pathogenicity island of FimX. Using a new multiplex PCR, fimX and the additional PAI-X genes were found to be highly associated with UPEC (144/173583.2 %), and more prevalent in UPEC of lower urinary tract origin (105/120587.5 %) than upper urinary tract origin (39/53574 %; P,0.05) or commensal isolates (28/78536 %; P¡0.0001). The Fim-like recombinase gene fimX is the only family member that has a significant association with UPEC compared to commensal isolates. Our results indicate PAI-X genes, including the type 1 pili regulator gene fimX, are highly prevalent among UPEC isolates and have a strong positive correlation with genomic virulence factors, suggesting a potential role for PAI-X in the extraintestinal pathogenic E. coli lifestyle.
UR - http://www.scopus.com/inward/record.url?scp=84881250647&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84881250647&partnerID=8YFLogxK
U2 - 10.1099/mic.0.066472-0
DO - 10.1099/mic.0.066472-0
M3 - Article
C2 - 23744903
AN - SCOPUS:84881250647
VL - 159
SP - 1606
EP - 1617
JO - Microbiology
JF - Microbiology
SN - 1350-0872
IS - 8
ER -