The type 1 pili regulator gene fimX and pathogenicity island PAI-X as molecular markers of uropathogenic Escherichia coli

Stacey L. Bateman, Ann E. Stapleton, Walter E. Stamm, Thomas M. Hooton, Patrick C. Seed

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Uropathogenic Escherichia coli (UPEC) fall within a larger group of isolates producing extraintestinal disease. UPEC express type 1 pili as a critical virulence determinant mediating adherence to and invasion into urinary tract tissues. Type 1 pili expression is under regulation by a family of site-specific recombinases, including FimX, which is encoded from a genomic island called PAI-X for pathogenicity island of FimX. Using a new multiplex PCR, fimX and the additional PAI-X genes were found to be highly associated with UPEC (144/173583.2 %), and more prevalent in UPEC of lower urinary tract origin (105/120587.5 %) than upper urinary tract origin (39/53574 %; P,0.05) or commensal isolates (28/78536 %; P¡0.0001). The Fim-like recombinase gene fimX is the only family member that has a significant association with UPEC compared to commensal isolates. Our results indicate PAI-X genes, including the type 1 pili regulator gene fimX, are highly prevalent among UPEC isolates and have a strong positive correlation with genomic virulence factors, suggesting a potential role for PAI-X in the extraintestinal pathogenic E. coli lifestyle.

Original languageEnglish (US)
Pages (from-to)1606-1617
Number of pages12
JournalMicrobiology (United Kingdom)
Volume159
Issue number8
DOIs
StatePublished - Aug 1 2013

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Uropathogenic Escherichia coli
Genomic Islands
Regulator Genes
Urinary Tract
Genes
Recombinases
Multiplex Polymerase Chain Reaction
Virulence Factors
Virulence
Life Style

ASJC Scopus subject areas

  • Microbiology

Cite this

Bateman, Stacey L. ; Stapleton, Ann E. ; Stamm, Walter E. ; Hooton, Thomas M. ; Seed, Patrick C. / The type 1 pili regulator gene fimX and pathogenicity island PAI-X as molecular markers of uropathogenic Escherichia coli. In: Microbiology (United Kingdom). 2013 ; Vol. 159, No. 8. pp. 1606-1617.
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The type 1 pili regulator gene fimX and pathogenicity island PAI-X as molecular markers of uropathogenic Escherichia coli. / Bateman, Stacey L.; Stapleton, Ann E.; Stamm, Walter E.; Hooton, Thomas M.; Seed, Patrick C.

In: Microbiology (United Kingdom), Vol. 159, No. 8, 01.08.2013, p. 1606-1617.

Research output: Contribution to journalArticle

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