The use and evaluation of 2+2 photoaddition in immobilization of oligonucleotides on a three dimensional hydrogel matrix

R. Elghanian; Y. Xu; J. McGowen; M. Seithoff; C. G. Liu; J. Winick; N. Fuller; R. Ramakrishnan; A. Beuhler; T. Johnson; A. Mazumder; C. K. Brush

doi:10.1081/NCN-100002558

The use and evaluation of 2+2 photoaddition in immobilization of oligonucleotides on a three dimensional hydrogel matrix

R. Elghanian, Y. Xu, J. McGowen, M. Seithoff, C. G. Liu, J. Winick, N. Fuller, R. Ramakrishnan, A. Beuhler, T. Johnson, A. Mazumder, C. K. Brush^*

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Photochemical attachment of synthetic oligonucleotides on the three dimensional surface of a polyacrylamide based hydrogel was used in the specific detection of target oligonucleotides. Covalent attachment of the oligonucleotide to the hydrogel was mediated by the incorporation of a 2+2 photo-attachable functional group in both the hydrogel and the oligonucleotide probe. Expression and SNP assays were used to evaluate this platform.

Original language	English (US)
Pages (from-to)	1371-1375
Number of pages	5
Journal	Nucleosides, Nucleotides and Nucleic Acids
Volume	20
Issue number	4-7
DOIs	https://doi.org/10.1081/NCN-100002558
State	Published - Jan 1 2001

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Genetics

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Elghanian, R., Xu, Y., McGowen, J., Seithoff, M., Liu, C. G., Winick, J., Fuller, N., Ramakrishnan, R., Beuhler, A., Johnson, T., Mazumder, A., & Brush, C. K. (2001). The use and evaluation of 2+2 photoaddition in immobilization of oligonucleotides on a three dimensional hydrogel matrix. Nucleosides, Nucleotides and Nucleic Acids, 20(4-7), 1371-1375. https://doi.org/10.1081/NCN-100002558

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abstract = "Photochemical attachment of synthetic oligonucleotides on the three dimensional surface of a polyacrylamide based hydrogel was used in the specific detection of target oligonucleotides. Covalent attachment of the oligonucleotide to the hydrogel was mediated by the incorporation of a 2+2 photo-attachable functional group in both the hydrogel and the oligonucleotide probe. Expression and SNP assays were used to evaluate this platform.",

author = "R. Elghanian and Y. Xu and J. McGowen and M. Seithoff and Liu, {C. G.} and J. Winick and N. Fuller and R. Ramakrishnan and A. Beuhler and T. Johnson and A. Mazumder and Brush, {C. K.}",

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Elghanian, R, Xu, Y, McGowen, J, Seithoff, M, Liu, CG, Winick, J, Fuller, N, Ramakrishnan, R, Beuhler, A, Johnson, T, Mazumder, A & Brush, CK 2001, 'The use and evaluation of 2+2 photoaddition in immobilization of oligonucleotides on a three dimensional hydrogel matrix', Nucleosides, Nucleotides and Nucleic Acids, vol. 20, no. 4-7, pp. 1371-1375. https://doi.org/10.1081/NCN-100002558

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AU - Elghanian, R.

AU - Xu, Y.

AU - McGowen, J.

AU - Seithoff, M.

AU - Liu, C. G.

AU - Winick, J.

AU - Fuller, N.

AU - Ramakrishnan, R.

AU - Beuhler, A.

AU - Johnson, T.

AU - Mazumder, A.

AU - Brush, C. K.

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AB - Photochemical attachment of synthetic oligonucleotides on the three dimensional surface of a polyacrylamide based hydrogel was used in the specific detection of target oligonucleotides. Covalent attachment of the oligonucleotide to the hydrogel was mediated by the incorporation of a 2+2 photo-attachable functional group in both the hydrogel and the oligonucleotide probe. Expression and SNP assays were used to evaluate this platform.

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SN - 1525-7770

IS - 4-7

ER -

The use and evaluation of 2+2 photoaddition in immobilization of oligonucleotides on a three dimensional hydrogel matrix

Abstract

ASJC Scopus subject areas

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