The use and evaluation of 2+2 photoaddition in immobilization of oligonucleotides on a three dimensional hydrogel matrix

R. Elghanian, Y. Xu, J. McGowen, M. Seithoff, C. G. Liu, J. Winick, N. Fuller, R. Ramakrishnan, A. Beuhler, T. Johnson, A. Mazumder, C. K. Brush*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Photochemical attachment of synthetic oligonucleotides on the three dimensional surface of a polyacrylamide based hydrogel was used in the specific detection of target oligonucleotides. Covalent attachment of the oligonucleotide to the hydrogel was mediated by the incorporation of a 2+2 photo-attachable functional group in both the hydrogel and the oligonucleotide probe. Expression and SNP assays were used to evaluate this platform.

Original languageEnglish (US)
Pages (from-to)1371-1375
Number of pages5
JournalNucleosides, Nucleotides and Nucleic Acids
Volume20
Issue number4-7
DOIs
StatePublished - Jan 1 2001

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Genetics

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