The use of second-generation assays in pre- and posttransplant monitoring: the primed or second-degree MLC

We have been unable to confirm reports of detection of increased spontaneous or primary blastogenesis in PBL prior to rejection and in our experience, this has been more common with systemic infection. The present assay differs from the primary spontaneous blastogenic assay in that it is specific for altered cellular immunity to alloantigens. Changes can be detected in it, despite the long period in culture (9 days), that occur significantly in advance of functional deterioration to still allow for prospective increase in immunosuppression.