Thermal desorption behavior and binding properties of DNA bases and nucleosides on gold

Linette M. Demers, Mattias Östblom, Hua Zhang, Nak Han Jang, Bo Liedberg, Chad A. Mirkin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

221 Scopus citations

Abstract

A comparison of the binding of DNA bases (adenine, cytosine, guanine, and thymine) and nucleosides (2′deoxyadenosine, 2′deoxycytidine, 2′deoxyguanosine, and thymidine) to gold thin films is presented. Desorption of monolayer/submonolayer and multilayer films of the adsorbates on gold studied via temperature-programmed desorption (TPD) and reflection-absorption infrared (RAIR) spectroscopy reveals that there are major differences in the binding affinities of the different bases to gold, for example, thymine ΔHdes = 111 ± 2 kJ/mol compared to guanineΔHdes = 146 ± 2 kJ/mol. The differences can be rationalized by molecular structures of the bases and their binding modes to gold surfaces deduced from IR data. Similar trends in desorption energies, shifted to lower desorption energy by more than 10 kJ/mol, are observed for deoxynucleoside layers on gold thin films.

Original languageEnglish (US)
Pages (from-to)11248-11249
Number of pages2
JournalJournal of the American Chemical Society
Volume124
Issue number38
DOIs
StatePublished - Sep 25 2002

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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