Thermal desorption behavior and binding properties of DNA bases and nucleosides on gold

Linette M. Demers; Mattias Östblom; Hua Zhang; Nak Han Jang; Bo Liedberg; Chad A. Mirkin

doi:10.1021/ja0265355

Thermal desorption behavior and binding properties of DNA bases and nucleosides on gold

Linette M. Demers, Mattias Östblom, Hua Zhang, Nak Han Jang, Bo Liedberg, Chad A. Mirkin^*

^*Corresponding author for this work

Robert H. Lurie Comprehensive Cancer Center

Research output: Contribution to journal › Article › peer-review

256 Scopus citations

Abstract

A comparison of the binding of DNA bases (adenine, cytosine, guanine, and thymine) and nucleosides (2′deoxyadenosine, 2′deoxycytidine, 2′deoxyguanosine, and thymidine) to gold thin films is presented. Desorption of monolayer/submonolayer and multilayer films of the adsorbates on gold studied via temperature-programmed desorption (TPD) and reflection-absorption infrared (RAIR) spectroscopy reveals that there are major differences in the binding affinities of the different bases to gold, for example, thymine ΔH_des = 111 ± 2 kJ/mol compared to guanineΔH_des = 146 ± 2 kJ/mol. The differences can be rationalized by molecular structures of the bases and their binding modes to gold surfaces deduced from IR data. Similar trends in desorption energies, shifted to lower desorption energy by more than 10 kJ/mol, are observed for deoxynucleoside layers on gold thin films.

Original language	English (US)
Pages (from-to)	11248-11249
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	124
Issue number	38
DOIs	https://doi.org/10.1021/ja0265355
State	Published - Sep 25 2002

ASJC Scopus subject areas

General Chemistry
Biochemistry
Catalysis
Colloid and Surface Chemistry

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title = "Thermal desorption behavior and binding properties of DNA bases and nucleosides on gold",

abstract = "A comparison of the binding of DNA bases (adenine, cytosine, guanine, and thymine) and nucleosides (2′deoxyadenosine, 2′deoxycytidine, 2′deoxyguanosine, and thymidine) to gold thin films is presented. Desorption of monolayer/submonolayer and multilayer films of the adsorbates on gold studied via temperature-programmed desorption (TPD) and reflection-absorption infrared (RAIR) spectroscopy reveals that there are major differences in the binding affinities of the different bases to gold, for example, thymine ΔHdes = 111 ± 2 kJ/mol compared to guanineΔHdes = 146 ± 2 kJ/mol. The differences can be rationalized by molecular structures of the bases and their binding modes to gold surfaces deduced from IR data. Similar trends in desorption energies, shifted to lower desorption energy by more than 10 kJ/mol, are observed for deoxynucleoside layers on gold thin films.",

author = "Demers, {Linette M.} and Mattias {\"O}stblom and Hua Zhang and Jang, {Nak Han} and Bo Liedberg and Mirkin, {Chad A.}",

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T1 - Thermal desorption behavior and binding properties of DNA bases and nucleosides on gold

AU - Demers, Linette M.

AU - Östblom, Mattias

AU - Zhang, Hua

AU - Jang, Nak Han

AU - Liedberg, Bo

AU - Mirkin, Chad A.

PY - 2002/9/25

Y1 - 2002/9/25

N2 - A comparison of the binding of DNA bases (adenine, cytosine, guanine, and thymine) and nucleosides (2′deoxyadenosine, 2′deoxycytidine, 2′deoxyguanosine, and thymidine) to gold thin films is presented. Desorption of monolayer/submonolayer and multilayer films of the adsorbates on gold studied via temperature-programmed desorption (TPD) and reflection-absorption infrared (RAIR) spectroscopy reveals that there are major differences in the binding affinities of the different bases to gold, for example, thymine ΔHdes = 111 ± 2 kJ/mol compared to guanineΔHdes = 146 ± 2 kJ/mol. The differences can be rationalized by molecular structures of the bases and their binding modes to gold surfaces deduced from IR data. Similar trends in desorption energies, shifted to lower desorption energy by more than 10 kJ/mol, are observed for deoxynucleoside layers on gold thin films.

AB - A comparison of the binding of DNA bases (adenine, cytosine, guanine, and thymine) and nucleosides (2′deoxyadenosine, 2′deoxycytidine, 2′deoxyguanosine, and thymidine) to gold thin films is presented. Desorption of monolayer/submonolayer and multilayer films of the adsorbates on gold studied via temperature-programmed desorption (TPD) and reflection-absorption infrared (RAIR) spectroscopy reveals that there are major differences in the binding affinities of the different bases to gold, for example, thymine ΔHdes = 111 ± 2 kJ/mol compared to guanineΔHdes = 146 ± 2 kJ/mol. The differences can be rationalized by molecular structures of the bases and their binding modes to gold surfaces deduced from IR data. Similar trends in desorption energies, shifted to lower desorption energy by more than 10 kJ/mol, are observed for deoxynucleoside layers on gold thin films.

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Thermal desorption behavior and binding properties of DNA bases and nucleosides on gold

Abstract

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