Thermodynamic properties of the kinesin neck-region docking to the catalytic core

S. Rice, Y. Cui, C. Sindelar, N. Naber, M. Matuska, R. Vale, Roger Cooke*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

115 Scopus citations

Abstract

Kinesin motors move on microtubules by a mechanism that involves a large, ATP-triggered conformational change in which a mechanical element called the neck linker docks onto the catalytic core, making contacts with the core throughout its length. Here, we investigate the thermodynamic properties of this conformational change using electron paramagnetic resonance (EPR) spectroscopy. We placed spin probes at several locations on the human kinesin neck linker and recorded EPR spectra in the presence of microtubules and either 5′-adenylylimidodiphosphate (AMPPNP) or ADP at temperatures of 4-30°C. The free-energy change (ΔG) associated with AMPPNP-induced docking of the neck linker onto the catalytic core is favorable but small, about 3 kJ/mol. In contrast, the favorable enthalpy change (ΔH) and unfavorable entropy change (TΔS) are quite large, about 50 kJ/mol. A mutation in the neck linker, V331A/N332A, results in an unfavorable ΔG for AMPPNP-induced zipping of the neck linker onto the core and causes motility defects. These results suggest that the kinesin neck linker folds onto the core from a more unstructured state, thereby paying a large entropic cost and gaining a large amount of enthalpy.

Original languageEnglish (US)
Pages (from-to)1844-1854
Number of pages11
JournalBiophysical Journal
Volume84
Issue number3
DOIs
StatePublished - Mar 1 2003

ASJC Scopus subject areas

  • Biophysics

Fingerprint Dive into the research topics of 'Thermodynamic properties of the kinesin neck-region docking to the catalytic core'. Together they form a unique fingerprint.

Cite this