Abstract
We describe the synthesis of small (2-nm diameter) gold nanoparticles densely functionalized with thiolated DNA (DNA-Au NPs) and a method to separate these particles from excess free DNA after synthesis. The separation method utilizes the thermodynamically enhanced binding properties of 2-nm DNA-Au NPs, compared to free excess DNA, to selectively hybridize these small particles to larger (15-nm diameter) DNA-Au NPs and form aggregates that can be isolated by simple centrifugation. These 2-nm DNA-Au NPs are obtained in a 46% overall yield, have a high surface coverage of DNA (64.8 ± 6.4 pmol/cm2), and as a result, exhibit increased melting temperatures and cooperative melting properties.
Original language | English (US) |
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Pages (from-to) | 5430-5431 |
Number of pages | 2 |
Journal | Journal of the American Chemical Society |
Volume | 130 |
Issue number | 16 |
DOIs | |
State | Published - Apr 23 2008 |
ASJC Scopus subject areas
- General Chemistry
- Biochemistry
- Catalysis
- Colloid and Surface Chemistry